成熟瘢痕组织与正常皮肤脱细胞真皮基质的生物学性能比较  

Biological properties of acellular dermal matrix derived from mature scar tissue versus normal skin

作　　者：王杨杨[1] 李小静[2] 卜寿山[3] 

机构地区：[1]安徽省淮北市人民医院,安徽省淮北市235000 [2]安徽医科大学第一附属医院整形外科,安徽省合肥市230032 [3]江苏省人民医院,江苏省南京市210029

出　　处：《中国组织工程研究》2016年第30期4496-4502,共7页Chinese Journal of Tissue Engineering Research

基　　金：江苏省自然科学基金面上项目(BK2011844)~~

摘　　要：背景:临床上大面积瘢痕患者需要进行多次手术切除瘢痕,这种切除的瘢痕组织是否可再利用成为研究的重点。目的:比较成熟瘢痕组织与正常皮肤脱细胞真皮基质的组织结构、生物力学性能及生物相容性等指标。方法:(1)取人成熟瘢痕组织和瘢痕周围正常皮肤,分别制备脱细胞真皮基质,进行组织学及扫描电镜观察,利用万能拉力试验机进行生物力学实验;(2)将成熟瘢痕组织脱细胞真皮基质与正常皮肤脱细胞真皮基质分别与成纤维细胞共培养10 d,绘制细胞生长曲线;(3)将成熟瘢痕组织脱细胞真皮基质与正常皮肤脱细胞真皮基质分别置入S D大鼠背部皮下,置入后4,8,12周进行组织学观察。结果与结论:(1)两组脱细胞真皮基质内无任何细胞成分,可见许多空隙,成熟瘢痕组织脱细胞真皮基质胶原纤维较正常皮肤松散,排列稍显紊乱;成熟瘢痕组织脱细胞真皮基质的生物力学性能与正常皮肤脱细胞真皮基质相近,有足够的弹性和强度;(2)两种脱细胞真皮基质上的细胞生长状态无明显差异,生长曲线基本一致;(3)置入4周时,成熟瘢痕组织脱细胞真皮基质组可观察到较多炎细胞浸润,正常皮肤脱细胞真皮基质组仅见少量炎症细胞浸润,随时间的延长,炎性反应逐渐消失,两组胶原纤维排列规整,植入物内均有小血管长入;(4)结果表明,瘢痕组织脱细胞真皮基质的组织结构、生物力学性能及生物相容性均十分接近人体正常皮肤。BACKGROUND： It is necessary to carry out multiple operations to remove the scar in patients with large area of scar, and whether the scar tissue can be recycled has become the focus of the study. OBJECTIVE： To compare the tissue structure, biomechanical properties and biocompatibility of the acellular dermal matrix of mature scar tissue and normal skin. METHODS： The acellular dermal matrix was prepared from the human mature scar tissue and the normal skin around the scar. Subsequently, histological and scanning electron microscope observations were performed, and biomechanical properties were detected using universal tensile testing machine. Then, the acellular dermal matrix from mature scar tissue and normal skin was co-cultured with fibroblasts for 10 days, respectively, and the cell growth curve was drawn. Additionally, the acellular dermal matrix from mature scar tissue and normal skin was subcutaneously implanted into the dorsal tissue of Sprague-Dawley rats, respectively and histological observation was conducted at 4, 8 and 12 weeks after implantation. RESULTS AND CONCLUSION： There were many gaps but no cellular components in the acellular dermal matrix, in both two groups. Collagen fibers of the acellular dermal matrix derived from mature scar were looser than that of the normal skin, and arranged slightly irregularly; the biomechanical properties of the acellular dermal matrix derived from mature scar were similar to that of the normal skin, which exhibited appropriate flexibility and strength. There was no significant difference in the growth state of the two kinds of aceilular dermal matrix, and the growth curve was basically consistent. After 4 weeks of implantation, more inflammatory cells infiltration could be found in the mature scar group, and in contrast, only a few infiammatory cells infiltration appeared in the normal skin group, These inflammatory reactions disappeared with time in both two groups. Besides, collagen fibers arranged in neat, and small vessels grew into the implants in b

关 键 词：瘢痕 皮肤 生物力学 组织工程 生物材料 材料相容性 脱细胞真皮基质 成熟瘢痕组织 组织结构 生物相容性 江苏省自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]