microRNA-155、microRNA．222与丝裂原活化蛋白激酶信号通路在室间隔缺损中的作用  

The role of microRNA - 155, microRNA - 222 and mitogen - activated protein kinase signaling pathway in ven- tricular septai defect

作　　者：纪龙[1] 刘连波[2] 刘一志[1] 孙强[1] 吴雪嵩 李栋[1] 

机构地区：[1]泰山医学院公共卫生学院,271000 [2]泰安市妇幼保健院小儿心脏外科,271000

出　　处：《中华实用儿科临床杂志》2016年第13期1027-1030,共4页Chinese Journal of Applied Clinical Pediatrics

基　　金：山东省自然科学基金（ZR2011HM018）;山东省医药卫生科技发展计划项目（2014WS0193,2013WSB31012）

摘　　要：目的探讨microRNA（miR）-155和miR-222在室间隔缺损（VSD）患者血浆中的表达变化及临床意义，对其可能的分子调控机制进行初步分析。方法选取2012年8月至2013年6月在济南市儿童医院心血管外科就诊和治疗的VSD患者20例（病例组），同期骨外科骨折患者15例（对照组）。反转录实时定量PCR（RT—qPCR）测定各组研究对象血浆miR-155和miR-222的表达水平。利用miRNA靶基因预测数据库Tar-getScan、mirbase及Miranda对miR-155和miR-222的靶基因进行预测，进而通过Pathway分析推测miRNA在VSD中的分子调控通路。结果与对照组相比，VSD患者血浆miR-155表达分别下调0．45倍（P=0．033）和0．51倍（P〈0．001）；miR-155和miR-222的下游调控靶基因分别有74个和50个；Pathway分析得到7个相关信号通路，其中包括与心脏发育密切相关的信号通路，如丝裂原活化蛋白激酶（MAPK）信号通路。结论VSD患者血浆miR-155和miR-222表达显著降低，其调控的靶基因与心脏发育密切相关的信号通路（MAPK信号通路）有关，提示miR-155和miR-222可作为预测VSD发病风险的独立评价指标。Objective To explore the expression and clinical significance of microRNA （miR） - 155 and miR -222 in plasma of patients with ventricular septal defect（ VSD）, and to analyze the possible mechanism. Methods A total of 20 children with VSD who received treatment at the Department of Cardiovascular Surgery from August 2012 to June 2013 were enrolled （the VSD group） and 15 patients with fracture （the control group）. The plasma miR - 155 and miR -222 expression levels were measured by real - time quantitative reverse transcription - polymerase chain reaction （ RT - qPCR）. The potential target genes of miR - 155 and miR -222 were predicted by using 3 current- ly available prediction programs, including TargetScan, mirbase and Miranda, and the signaling pathway of miRNA was predicted by Pathway - express analysis. Results Compared with the control group, the expression levels of miR - 155 （P = 0. 033 ） and miR - 222 （ P 〈 0.001 ） in the VSD group decreased significantly ; miR - 155 and miR - 222 predic- ted target genes included 74 and 50, respectively. The Pathway - express analysis indicated that 7 signaling pathways played important roles in the occurrence of fetal VSD, including signaling pathways for heart development, such as：mito- gen -activated protein kinase （MAPK） signaling pathway. Conclusions The expression levels of plasma miR - 155 and miR -222 in VSD were significantly decreased. The target genes were related to signaling pathways for heart deve- lopment （ MAPK signaling pathway） ,which indicates that miR - 155 and miR - 222 may be involved in the pathological process of VSD, and may serve as an independent evaluation indicator for the diagnosis of VSD.

关 键 词：微小RNA 室间隔缺损 丝裂原活化蛋白激酶信号通路 

分 类 号：R725.4[医药卫生—儿科]