促性腺激素释放激素激动剂预处理对性成熟雌鼠调节性T细胞的影响  

Effects of gonadotropin-releasing hormone agonist pretreatment on regulatory T cells in mature female mice

作　　者：彭周雨 温彦[1] 赵婷婷[3] 傅晓岚[3] 贺丽人[1] 徐萍萍[1] 何畏[1] 

机构地区：[1]第三军医大学西南医院妇产科,重庆400038 [2]武警四川总队成都医院外科,成都610041 [3]第三军医大学全军免疫研究所,重庆400038

出　　处：《免疫学杂志》2016年第8期674-680,共7页Immunological Journal

摘　　要：目的 探讨促性腺激素释放激素激动剂（gonadotropin releasing hormone agonist,GnRHa）预处理对性成熟雌鼠调节性T细胞（regulatory T cells,Treg）的影响。方法 30只动情周期正常的7-8周未交配KM雌鼠随机分成5组,第1组连续9 d每日注射醋酸曲普瑞林1次,第2组连续9d每日注射醋酸曲普瑞林后,在第9天注射重组人促卵泡激素1次,第10天注射绒毛膜促性腺激素1次,第3组、4组分别与第1组、2组对应,用生理盐水代替醋酸曲普瑞林,其余处理不变,第5组无药物干预。流式细胞学检测各组外周血、脾脏T淋巴细胞亚群及Treg比例;并分别从前4组中随机选取4只小鼠留取子宫内膜,用实时荧光定量PCR方法检测子宫内膜Foxp3 mRNA含量。结果 流式细胞技术检测结果发现,外周血T淋巴细胞亚群及Treg比例各组比较无明显差异。比较脾脏T淋巴细胞亚群及Treg比例发现,与第5组比较,第1、2、3、4组CD3＋比例下降（P〈0.05）,第2组CD4^＋比例下降（P〈0.05）;第2组CD4^＋CD25^＋Foxp3^＋Treg比例高于第4、5组（P〈0.05）;CD8^＋比例在各组间未见明显差异。实时荧光定量PCR检测结果发现,与第3组比较,第1组子宫内膜Foxp3 mRNA升高（P〈0.05）;与第4组比较,第2组子宫内膜Foxp3 mRNA升高（P〈0.05）。结论 GnRHa预处理后促排卵,可提高脾脏Treg比例及子宫内膜Foxp3 mRNA含量,可能提高机体免疫耐受水平。This study designed to investigate the effects of gonadotropin-releasing hormone agonist （GnRHa） pretreatment on regulatory T cells （Treg） in mature female mice. Total of 30 unmated （7-8 weeks old） KM female mice with normal estrous cycle were randomly divided into 5 groups： group 1 received triptorelin acetate injection once daily for 9 consecutive days; group 2 also received triptorelin acetate injection once daily for 9 consecutive days, then received recombinant human follicle-stimulating hormone （r-hFSH） and choriogonadotropin （HCG） on the 9 th and 10 th days respectively; group 3 and 4 were corresponded with those of groups 1 and 2 respectively, but used saline instead of triptorelin acetate; group 5 was normal control with no drug intervention. Flow cytometry technology was used to evaluate the T cell subpopulation and Treg in peripheral blood and spleen of each group. RT-PCR was implemented to detect the expression of Foxp3 mRNA in the endometrium. Data showed that there was no significant difference in the proportion of T cell subpopulation and Treg in peripheral blood among the groups. But, the proportion of CD3^＋T cell in the group 5 was lower than that of the other four groups （P〈0.05）; the proportion of CD4^＋T cell in group 2 were lower than that of the group 5 （P〈0.05）;the proportion of CD4^＋CD25^＋Foxp3^＋T cell in group 2 was higher than that of the group 4 and group 5（P〈0.05）. No significant difference in proportion of CD8^＋T lymphocyte was found among the groups. Furthermore, the expression of Foxp3 mRNA in the endometrium of the group 1 was higher than that of the group 3 （P〈0.05）, and the expression of Foxp3 mRNA in the endometrium of the group 2 was higher than that of the group 4（P〈0.05）. In conclusion, stimulating ovarian with the pretreatment of GnRHa could increase the proportion of CD4^＋CD25^＋Foxp3^＋T cell in spleen and the expression of Foxp3 mRNA in endometrium, and then upregulates the function of immune tolerance.

关 键 词：促性腺激素释放激素激动剂 调节性T细胞 FOXP3 

分 类 号：R965[医药卫生—药理学]