雏鸡免疫器官中chMDA5的表达与定位  

The Expression and Location of chMDA5 in Chick Immune Organs

作　　者：栾亚男 葛铭 李广兴[1] 谢婉秋 杨贵君[1] 张瑞莉[1] 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030

出　　处：《畜牧兽医学报》2016年第7期1480-1487,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31272533)

摘　　要：为研究鸡模式识别受体MDA5（chMDA5）在雏鸡免疫器官中的表达与定位,分别于1、7、14、21、28、31、35、42、45、49日龄时从50只健康海兰白蛋鸡中随机选取5只,采取法氏囊、胸腺及脾,应用实时荧光定量PCR、Western blot及间接免疫荧光技术检测雏鸡免疫器官中chMDA5的表达情况。结果发现,在各日龄雏鸡法氏囊、胸腺及脾内均检测到chMDA5转录;在法氏囊淋巴滤泡的皮质、胸腺小叶的皮质和髓质及脾的白髓和红髓部位均检测到chMDA5阳性细胞;法氏囊和胸腺组织中chMDA5蛋白的表达规律较为一致,即在1～7日龄呈上升趋势并在7日龄达到峰值,随后下调,在14～35日龄时呈波动变化,之后逐渐上调并趋于稳定;脾中chMDA5蛋白表达以14d左右为一个周期变化,随着日龄增大其表达量在总体上趋于上调。结果表明,chMDA5在雏鸡免疫器官中均有表达,且不同日龄之间的表达存在差异。本研究为进一步探究chMDA5与雏鸡免疫功能相关性提供了科学的试验依据。The objective of the present study was to examine the expression and location of chick melanoma differentiation associated gene 5（chMDA5）in chick immune organs.Fifty health Highland white chicks were euthanized to collect bursa of Fabricius,thymus and spleen at 1,7,14,21,28,31,35,42,45,49-day-old,separately.Then,real-time fluorescence quantitative PCR,Western blot and IFA techniques were used to detect the expression of chMDA5 in chick immune organs.The results showed that the chMDA5 mRNA were examined in bursa of Fabricius,thymus and spleen at each age,chMDA5＋cells were detected in the cortex of bursal lymphoid follicles,the cortex and medulla of thymus lobule,the white pulp and red pulp of spleen.The expression pattern of chMDA5 protein in bursa of Fabricius and thymus tissue was consistent,which showed an upward trend in 1to 7-day-old chicks and reached peak at 7-day-old,and then went down in 14 to 35-day-old with a fluctuation trend,then gradually increase and stabilized.In spleen,the protein expression of chMDA5 was increased with the increasing age in a period of about 14 days.The results indicated that the chMDA5 were expressed in chick immune organs,and there were differences in expression of chMDA5 with different ages.This study provide a scientific test basis for further exploration about the correlation between chMDA5 and chick immune function.

关 键 词：chMDA5 雏鸡 免疫器官 表达 定位 

分 类 号：S852.4[农业科学—基础兽医学]