黑曲霉发酵白术对小鼠胸腺指数、脾指数及脾T淋巴细胞增殖的影响  

Effect of Aspergillus niger-Fermented Atractylodes macrocephala Koidz on the Spleen Index,Thymus Index and T Lymphocyte Proliferation of Mice

作　　者：孙向婉 王芸[1] 王鑫[1] 刘晓晔[2] 穆祥[1] 董虹[1] 

机构地区：[1]北京农学院兽医学(中医药)北京市重点实验室,北京102206 [2]中国农业大学动物医学院,北京100193

出　　处：《畜牧兽医学报》2016年第7期1502-1510,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31572558;31272144);北京市科技新星(Z141105001814041);北京市农业科技项目(20150126)

摘　　要：旨在研究黑曲霉发酵白术对小鼠免疫功能的影响。利用环磷酰胺建立小鼠免疫抑制模型,检测黑曲霉发酵白术对小鼠胸腺指数、脾指数和脾T淋巴细胞增殖的影响。通过高效液相色谱技术(HPLC)检测白术发酵前后的有效成分变化。并通过正交试验筛选了提高白术有效成分含量的最佳发酵条件。试验结果表明:(1)黑曲霉发酵后的白术能有效提高小鼠免疫功能,其中发酵白术低、中、高剂量(0.5、1、2g·kg^(-1))均能显著提高胸腺指数(P<0.05);而中剂量发酵白术能显著提高脾指数及小鼠脾T淋巴细胞增殖(P<0.05);(2)HPLC检测黑曲霉菌发酵白术后白术内酯Ⅰ的含量升高;(3)确立提高有效成分白术内酯Ⅰ含量的最佳发酵条件为接菌量8%,含水量40%,发酵时间96h,此条件下白术内酯Ⅰ含量是原来的1.4倍。试验进一步揭示黑曲霉发酵白术可通过发酵后白术内酯Ⅰ含量的升高来增强小鼠免疫功能。This study aimed to study the effect of Aspergillusniger-fermented Atractylodes macrocephala Koidz on the immune function of mice.An immunosuppression mice model（cyclophosphamide）was utilize to study the effect of fermented Atractylodes macrocephala Koidz on spleen index,thymus index and T lymphocyte proliferation.High performance liquid chromatography（HPLC）was used to analyze the changes in the active omponents of Atractylodes macrocephala Koidz with or without fermentation by Aspergillus niger.Orthogonal experiment was conducted to select the best conditions.The results showed that：1）The low,middle and high dose groups（0.5,1,2g·kg^（-1））of fermented Atractylodesmacrocephala Koidz can effectively increase thymus index（P 〈0.05）.In addition,middle dosage group of fermented Atractylodesmacrocephala Koidz can effectively increase spleen index and T lymphocyte proliferation（P 〈0.05）;2）The results of HPLC showed that the content of atractylenolideⅠ was effectively increased in the Atractylodes macrocephala Koidz processed by Aspergillus niger compared with unfermented At-ractylodes macrocephala Koidz;3）The best fermentation conditions were as follows：inoculum concentration was 8%;water content was 40%;fermentation time was 96 h,under the optimized conditions,the content of atractylenolide Ⅰ was 1.4times of those in raw Atractylodes macrocephala Koidz.These results indicated that fermented Atractylodes macrocephala Koidz could improve immune function of mice by increasing the content of atractylenolideⅠ.

关 键 词：黑曲霉 发酵白术 免疫功能 白术内酯I 

分 类 号：S853.73[农业科学—临床兽医学]