miR-92a对大鼠骨髓源性内皮祖细胞功能的影响  

Effects of miR-92a on the Functions of Endothelial Progenitor Cells Isolated from Rat Bone Marrow

作　　者：宿云娟 孙玉聪[1] 楚海荣[2] 李宏[2] 崔晓栋[2] 王钢[3] 成敏[2] 

机构地区：[1]潍坊医学院生物科学与技术学院,潍坊261053 [2]潍坊医学院医学研究实验中心,潍坊261053 [3]潍坊医学院认知神经研究室,潍坊261053

出　　处：《中国细胞生物学学报》2016年第6期676-681,共6页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:31570941;31270993;30900290);国家教育部"新世纪优秀人才计划"资助项目(批准号:NCET-10-0922);山东省自然科学基金(批准号:ZR2013CQ032;ZR2014JL018);潍坊医学院大学生科技创新基金(批准号:KX2014015)资助的课题~~

摘　　要：该文探讨了miR．92a对大鼠骨髓源性内皮祖细胞（endothelial progenitor cells．EPCs）迁移、黏附、增殖以及血管形成能力等的影响。采用密度梯度离心法分离大鼠骨髓来源的单核细胞，体外培养于含10％胎牛血清的EGM－2MV完全培养基中。利用Lipofectamine TM3000分别将miR－92a模拟物（miR-92amimics）、miR－92a抑制剂（miR－92ainhibitor）及其对应的对照组（negativecontr01．NC）转染入第3～5代EPCs，即晚期EPCs。采用改良Boyden小室、黏附实验、CCK－8及Matrigel胶分别检测EPCs迁移、黏附、增殖及体外成血管能力。miR－92a模拟物明显抑制了EPCs的迁移和体外成血管能力（P〈0．05），但不影响EPCs的黏附和增殖能力；则miR－92a4抑剂则促进了EPCs的迁移和体外成血管能力（P〈0．05），亦不影响EPcs的黏附和增殖能力。结果表明，miR－92a可抑制EPCs的迁移及体外成血管能力。该研究结果将为临床以miR-92a和EPCs为靶点防治动脉粥样硬化性心血管疾病提供理论及实验依据。This article aimed to investigate the effects of miR-92a on the migration, adhesion, proliferation and angiogenesis of endothelial progenitor cells isolated from rat bone marrow. Mononuclear cells were isolated by density gradient centrifugation from rat bone marrow and then cultured with EGM-2MV containing 10% fetal bovine serum. The miR-92a mimics, miR-92a inhibitors and their corresponding negative controls （NC） were transfected into 3-5 generations EPCs, namely late EPCs, by LipofectamineTM 3000, respectively. Modified Boyden chamber, adhesion assay, CCK-8 assay and Matrigel were employed to study EPCs migration, adhesion, proliferation and angiogenesis in vitro, miR-92a mimics significantly inhibited EPCs migration and angiogenesis in vitro （P〈0.05）, but had no effect on EPCs adhesion and proliferation. However, miR-92a inhibitor obviously promoted EPCs migration and angiogenesis in vitro （P〈0.05）. miR-92a inhibits the migration and angiogenesis of EPCs in vitro. The results may provide new clinical strategies toward the prevention and/or treatment of cardiovascular diseases by targeting miR-92a and EPCs.

关 键 词：内皮祖细胞 miR-92a 迁移 增殖 血管形成 

分 类 号：R54[医药卫生—心血管疾病]