大肠杆菌SeqA蛋白质结构与功能  

Structure and Function of the Escherichia coli SeqA Protein

作　　者：乌日汗[1] 梁燕[1] 李静[1] 莫日根[1] 

机构地区：[1]内蒙古大学生命科学学院,呼和浩特010021

出　　处：《中国细胞生物学学报》2016年第6期721-728,共8页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:31360208)资助的课题~~

摘　　要：大肠杆菌Seq A蛋白质是染色体复制起始负调控因子。在体内,Seq A主要以四聚体或多聚体形式存在,有N-端多聚化结构域和C-端DNA结合结构域。大肠杆菌复制原点(the origin of replication of the Escherichia coli chromosome,ori C)有11个GATC位点,新复制的ori C处于半甲基化状态,其中相邻的两个半甲基化GATC是Seq A特异性结合靶位点。Seq A通过结合新复制的半甲基化ori C来抑制复制起重新发生,从而使ori C隔绝(sequestration)。由Seq A介导的ori C隔绝是抑制同一个细胞周期中复制起始重新发生的机制之一。Seq A不仅是复制起始调控因子,也是一个转录因子,抑制或激活一些基因的表达。该文就Seq A蛋白质的结构与功能域特点,对DNA复制起始和基因表达调控机制以及细胞分裂中的作用作一综述。The SeqA protein is a negative factor for initiation of chromosomal replication in Escherichia coli. In vivo the SeqA molecules are found in a form of tetramer or multimers. The SeqA protein has the N-terminal ag- gregation and the C-terminal DNA-binding domains. The E. coli chromosomal origin for replication （oriC） contains 11 GATC sites and newly replicated oriC is hemimethylated. The site of two neighboured hemimethylated GATC is the best target of SeqA for binding. SeqA binds to hemimthylated GATC to prevent re-initiation of replication, namely the sequestration of oriC. Sequestration is one of the mechanisms to make sure that each origin is initiated only once per cell cycle. The SeqA protein is not only a regulatory factor for replication initiation but also a transcription factor, inhibiting or activating expression of some genes. In the paper, we reviewed the character of structural and functional domain of SeqA, and its roles in control for initiation of DNA replication, gene expression and cell division.

关 键 词：SeqA蛋白质 结构与功能域 DNA复制起始 隔绝 

分 类 号：Q78[生物学—分子生物学] Q51