吴茱萸碱对小鼠大脑及肩胛间棕色脂肪摄取^(18)F-FDG的影响  

Effect of evodiamine on ^(18)F-FDG uptake in mouse brain and interscapular brown adipose tissue

作　　者：冯悦[1] 钟萌[2] 张莉[1] 朱艳[1] 邱琳[1] 陈跃[1] 

机构地区：[1]泸州医学院附属医院核医学科,四川泸州646000 [2]泸州医学院附属医院药剂科,四川泸州646000

出　　处：《第三军医大学学报》2016年第15期1746-1749,共4页Journal of Third Military Medical University

基　　金：泸州医学院校级资金资助计划(2013ZRQN067);四川省医学重点学科建设项目(2008-17)~~

摘　　要：目的利用小动物PET/CT观察吴茱萸碱(evodiamine,EVO)对小鼠大脑及肩胛间棕色脂肪(interscapular brown adipose tissue,IBAT)糖代谢的影响。方法采用完全随机分组法将昆明小鼠分为5组,分别腹腔注射剂量为0(空白组)、125、250、375 mg/kg的EVO混悬液,30 min后腹腔注射^(18)F-氟代脱氧葡萄糖(18F-FDG),1 h后采用小动物PET/CT观察各组小鼠大脑及IBAT摄取^(18)F-FDG的情况,并测定其标准化摄取值(SUV)。结果 5组小鼠IBAT的SUV值分别为(0.81±0.08)、(0.53±0.06)、(1.30±0.17)、(1.70±0.10)和(0.74±0.21)。剂量为250 mg/kg和375 mg/kg时,IBAT的SUV值与空白组比较明显升高(P<0.05)。5组小鼠大脑SUV值分别为(1.43±0.15)、(2.20±0.20)、(2.80±0.20)、(3.33±0.15)和(4.00±0.25),随EVO剂量增加而升高,组间比较差异均有统计学意义(P<0.05)。结论 EVO可以增加小鼠IBAT和大脑的糖代谢。Objective To study the effect of evodiamine （EVO） on glucose metabolism of brain and interscapular brown adipose tissue （IBAT） in mice quantified by ^18F-fluoro-deoxyglucose （^18F-FDG） positron emission tomography （PET）/computed tomography （CT） scanning. Methods Kunming mice were randomized into 5 groups （each group n = 3） and intraperitoneal （i. p. ） injected with EVO doses of 0, 125, 250, 375 and 500 mg/kg, and 18F-FDG was given in 30 rain later. The uptake of 18F-FDG in the brain and IBAT were observed after 1 h and standard uptake value （SUV） were measured by micro-PET/CT. Flesults TheSUVs of IBAT were 0.81 ±0.08, 0.53 ±0.06, 1.30 ±0. 17, 1.70 ±0. 10 and 0.74 ±0.21 corresponding to EVO doses of 0, 125, 250, 375 and 500 mg/kg. Compared to the control, the increased SUVs in the groups of 250 and 375 mg/kg were significant （P 〈 0.05）. The SUVs of brain were 1.43 ± 0. 15, 2.20 ± 0.20, 2.80 ± 0.20, 3.33 ± 0.15 and 4.00 ± 0.25, in a dose-dependent manner. Difference between each 2 groups was statistically significant （P 〈 0.05）. Gonclusion EVO enhances glucose metabolism in mice IBAT and brain.

关 键 词：吴茱萸碱 肩胛间棕色脂肪 糖代谢 小动物PET/CT 

分 类 号：Q591.4[生物学—生物化学] R285.5[医药卫生—中药学]