跑台运动对高脂饮食诱导的肥胖大鼠骨骼肌肌联素表达的影响  

Effects of Treadmill Running on the Myonectin in Skeletal Muscle of Obese Rats

作　　者：张靓[1] 董泽源 马谨[1] 陈雪飞[1] 

机构地区：[1]北京师范大学体育与运动学院,北京100875

出　　处：《中国运动医学杂志》2016年第6期547-552,共6页Chinese Journal of Sports Medicine

基　　金：国家自然科学基金(31471137)

摘　　要：目的:观察跑台运动对高脂饮食诱导的肥胖大鼠骨骼肌肌联素(myonectin)表达的影响。方法:5周龄健康雄性SD大鼠随机分成对照组和高脂喂养组,分别进行普通饲料和高脂饲料喂养,12周后筛选肥胖大鼠16只,随机分为高脂安静组和高脂运动组。跑台训练持续10周,每周5次,每次60 min,跑速26 m/min。测定大鼠血脂及血浆自由脂肪酸水平,采用real-time PCR方法测定比目鱼肌、腓肠肌肌联素m RNA表达,比目鱼肌脂肪酸转位酶(fatty acid translocase,FAT/CD36)的m RNA表达。结果:与对照组相比,高脂饮食喂养大鼠体重和体脂显著增加(P<0.01),总甘油三酯、总胆固醇和低密度脂蛋白水平显著升高,高密度脂蛋白水平显著降低(P<0.05),血自由脂肪酸(free fatty acid,FFA)水平显著升高(P<0.05)。比目鱼肌和腓肠肌肌联素m RNA表达水平显著升高(P<0.05),比目鱼肌CD36表达显著上调(P<0.05)。与高脂饮食组大鼠相比,10周跑台运动大鼠体重显著降低(P<0.01),血脂改善,血FFA水平显著降低(P<0.05)。此外,比目鱼肌和腓肠肌肌联素m RNA表达显著下调(P<0.05),比目鱼肌CD36表达显著降低(P<0.05)。相关性分析发现,骨骼肌肌联素水平与血脂和血糖水平呈显著正相关(P<0.05)。结论:骨骼肌肌联素的表达与血脂、血糖水平显著相关,运动促进了血脂和血糖水平的降低,进而间接下调了骨骼肌肌联素的表达,提示肌联素可能不参与运动引起的自由脂肪酸水平的降低。Objective To observe the alteration of the myonectin expression in skeletal muscle of obese rats induced by high-fat diet. Methods 5-week-old male SD rats were randomly divided into control group and high-fat diet group. 16 obese rats after 12-week feeding with high-fat diet were recruited and then randomly divided into sedentary group and treadmill running group. The rats in treadmill running group underwent 60-minute treadmill running once per day at the intensity of 26 m/min,5 days per week for 10 weeks. The m RNA expression of myonectin and fatty acid translocase（FAT/CD36）level were detected by real-time PCR. Results The body weight and body fat increased（P〈0.01）,levels of triglyceride,cholesterol and low density lipoprotein elevated（P〈0.05）,high density lipoprotein decreased（P〈0.05）,level of free fatty acid（FFA）increased（P〈0.05）,and the m RNA levels of myonectin and CD36 in soleus and gastrocnemius increased（P〈0.05）in obese rats as compared with the control group. The body weight decreased（P〈0.01）,lipid profile improved,serum FFA content decreased（P〈0.05）and the m RNA level of myonectin and CD36 in muscle decreased（P〈0.05）in treadmill running group after 10-week exercise as compared with sedentary obese group. There was positive correlation between the expression of myonectin m RNA and serum triglyceride,fasting blood sugar and FFA（P〈0.05）. Conclusion Results showed that exercise improved blood sugar and lipid metabolism,and thus downregulated the myonectin m RNA level in muscle of obese rats indirectly,suggesting that myonectin might not involve in the exercise-induced decreased level of FFA.

关 键 词：跑台运动 肌联素 自由脂肪酸 肥胖 骨骼肌 

分 类 号：R87[医药卫生—运动医学]