Hort16A猕猴桃叶片直接再生不定芽研究  

Direct Adventitious Bud Regeneration from Leaves of Hort16A kiwifruit

作　　者：刘子花 李晓蓉[1] 杨玲[1] 王昌命[1] 

机构地区：[1]西南林业大学生命科学学院,云南昆明650224

出　　处：《林业调查规划》2016年第3期87-91,共5页Forest Inventory and Planning

基　　金：云南省教育厅项目"云南省优势特色重点建设学科‘生物学’"(50097505);"云南省高校林下生物资源保护及利用科技创新团队"(51400605)

摘　　要：通过外植体直接再生不定芽方式进行器官再生,不经过愈伤组织,能使无性系后代保持原品种的特性。以Hort16A猕猴桃叶片为外植体,以MS培养基为基本培养基,设计不同类型和浓度的植物生长调节剂组合对诱-导分化效果进行研究。结果表明,经过4~5周暗培养后外植体褐化程度最低;诱导不定芽分化的最适植物生长调节剂组合为1.5 mg/L 6-BA＋0.5 mg/L IBA,在该浓度下,不定芽分化率为100%,平均出芽个数为6.9。Organ regeneration from explant by direct adventitious bud regeneration could make the clones to maintain the characteristics of the original varieties,not through the way of callus. The effect of induced differentiation was studied in this paper,by using the leaves of Hort16 A as explants,MS medium as the basic culture medium,and designing the plant growth regulator combinations of different types and concentrations. The results showed that after 4 or 5 weeks of dark culture,the browning degree of explants was the lowest. And the optimum combinations of plant growth regulators for induction of adventitious bud regeneration was 1. 5mg / L 6- BA ＋ 0. 5mg / L IBA,under this concentration combination the differentiation rate of adventitious buds was 100%,average number of shoots was 6. 9.

关 键 词：Hort16A猕猴桃 叶片离体培养 不定芽 外植体 褐化现象 植物生长调节剂 

分 类 号：S663.4[农业科学—果树学] S723.132[农业科学—园艺学]