HPLC-ELSD同时测定参芪复方颗粒中皂苷类成分的含量  

Simultaneous Determination of Saponins in Shenqi Compound Granules by HPLC-ELSD

作　　者：单丽芳[1] 袁彩[1] 杨红梅[1] 曹蕾[1] 谈静[2] 高天[2] 袁强华[2] 宋英[2] 

机构地区：[1]成都中医药大学,成都610075 [2]成都中医药大学附属医院,成都610072

出　　处：《中国实验方剂学杂志》2016年第14期102-105,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：四川省科技支撑项目(2014SZ0140)

摘　　要：目的:建立同时测定参芪复方颗粒中人参皂苷Rg1,Re,Rb1和黄芪甲苷含量的方法,为该制剂的质量控制提供参考。方法:采用HPLC-ELSD,流动相水(A)-乙腈(B)梯度洗脱(0~3 min,81%A;3~20 min,81%~78%A;20~28 min,78%~77%A;28~33 min,77%~75%A;33~40 min,75%~65.7%A;40~52 min,65.7%~70%A;52~62 min,70%A),流速0.8 m L·min^(-1),柱温25℃,气体流量2.6 L·min^(-1),漂移管温度102℃。结果:人参皂苷Rg_1,Re,Rb_1和黄芪甲苷分别在0.196~2.976,0.207~3.138,0.191~2.895,0.190~2.879μg与色谱峰面积呈良好线性关系,平均回收率分别为99.27%(RSD 1.5%),99.33%(RSD 1.1%),99.32%(RSD 1.0%),99.27%(RSD 2.4%)。结论:该含量测定方法同时测定参芪复方颗粒中多种指标成分时,精密度高、重复性好,可用于该制剂的质量控制。Objective ： To develop a method for determining four kinds of saponins in Shenqi compound granules. Method： Quantitative analysis was carried out on a column of Phenomenex C18 （4.6 mm × 250 mm, 5 μm） by HPLC-ELSD with mobile phase of acetonitrile-water under a flow rate of 0. 8 mL · min-1 , column temperature was 25 ℃ , flow rate of carrier gas was 2.6 L .min -1 , drift tube temperature was 102 ℃. Result： Linear ranges of ginsenoside Rg1 , ginsenoside Re, ginsenoside Rb1 and astragaloside Ⅳ were 0. 196-2. 976, 0. 207-3. 138, 0. 191-2. 895, 0. 190-2. 879μg; their recoveries were 99.27% , 99.33% , 99.32% , 99.27% , RSDs were 1.5% , 1.1% , 1.0% and 2.4% , respectively. Conclusion： This method is accurate, reproducible and convenient for quality control over Shenqi compound granules.

关 键 词：参芪复方颗粒 人参皂苷RG1 人参皂苷RE 人参皂苷RB1 黄芪甲苷 

分 类 号：R284.1[医药卫生—中药学] R283.6[医药卫生—中医学]