壮骨伸筋胶囊对去势大鼠骨密度及骨代谢的影响  

Effect of Zhuanggu Shenjin Capsules on Bone Mineral Density and Bone Metabolism in Ovariectmized Rats

作　　者：解秀兰 孙晓丽 李丽娟 苏海峰 段重高 周亚伟 

机构地区：[1]中国医药科技出版社,北京100082 [2]北大世佳研究中心,北京100084

出　　处：《中国实验方剂学杂志》2016年第14期121-124,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：吉林省科技发展计划项目(20130302025YY)

摘　　要：目的:研究壮骨伸筋胶囊(Zhuanggu Shenjin capsules,ZSJ)对去卵巢骨质疏松大鼠骨密度及骨代谢的影响,并探讨其相关的作用机制。方法:将72只SD大鼠按随机分为假手术组(12只)和造模组(60只),模型组采用摘除大鼠双侧卵巢,将去卵巢大鼠随机分为模型组、阳性药结合雌激素组、壮骨伸筋胶囊高、中、低剂量组(5.42,2.71,1.36 g·kg^(-1)),每组12只。连续ig 13周,每周连续ig 6 d,实验结束,取血和骨组织,测定骨密度及血清中血钙(Ca),碱性磷酸酶(ALP),抗酒石酸酸性磷酸酶(TRAP),雌二醇(E2),骨钙素(BGP),降钙素(CT)的变化;甲苯胺蓝染色观察骨组织病理学。结果:与假手术组比较,模型组大鼠骨密度,血清E2含量明显降低,大鼠血清ALP,TRAP,BGP含量明显升高(P<0.05,P<0.01),子宫系数明显降低(P<0.01),骨组织病理学观察显示模型组骨小梁稀疏,骨小梁间有断裂并且分离,间隙增大,排列紊乱,骨板层结构疏松,骨质疏松特征明显;与模型组比较,壮骨伸筋胶囊高、中剂量组能够显著增加模型大鼠的骨密度(P<0.01),增加模型鼠血清E2含量(P<0.05),降低模型大鼠血清ALP,TRAP,BGP含量(P<0.05),但是对血钙和CT作用却不明显,骨组织病理学观察显示骨组织结构趋于完整。结论:壮骨伸筋胶囊能提高去卵巢骨质疏松大鼠骨密度,提高雌激素水平,改善骨代谢水平,从而起到防治骨质疏松的作用。Objective： To observe the effect of Zhuanggu Shenjin capsules （ZSJ） on bone metabolism and bone mineral density （BMD） in ovariectmized rats. Method： Totally 72 SD rats were randomly divided into sham-operated group （n = 12） and modeling group （n = 60）. Bilateral ovaries of rats were removed to establish the modeling group, and then the rats in modeling group were further ransomly divided into model group, positive drug ＋ estrogen group, and ZSJ high dose, middle dose and low dose groups （5.42, 2.71, 1.36 g.kg-1 ）. Each group included 12 rats. The rats received corresponding drug by ig administration for continuous 13 weeks, 6 days/week. After the experiment, blood and bone tissues were taken to detect BMD, blood calcium （Ca）, alkaline phosphatase （ALP）, tartrate resistant acid phosphatase （TRAP）, estradiol （E2）, bone gla protein （BGP） and calcitonin in blood serum. The bone tissue pathology was observed by toluidine blue staining. Result： As compared with sham- operated group, BMD level and serum E2 level were significantly decreased, while serum ALP, TRAP and BGP levels were significantly increased in rats of model group （P 〈 0.05, P 〈 0.01 ） , uterine coefficient was decreased significantly （P 〈0.01）, the bone tissue pathological observation showed that the trabecular bone was sparse in rats of model group, with fractures and separation between the trabecular bones, gaps enlarged with arrangement disorder, loose bone lamellar structure, and the characteristic of osteoporosis was obvious. As compared with the model group, ZSJ （high dose, middle dose） could significantly increase the BMD level of the rats （P 〈 0.01 ）, increase the level of E2 in blood serum （P 〈 0.05 ）, and reduce ALP, TRAP and BGP levels （P 〈 0.05）, but had no significant effect on the level of blood Ca and CT, the bone tissue pathological observation showed that the bone tissue structure was nearly complete. Conclusion： ZSJ can increase bone mineral density an

关 键 词：壮骨伸筋胶囊 骨密度 骨代谢指标 骨质疏松 

分 类 号：R285.5[医药卫生—中药学]