健脾温胃散对脾气虚胃炎大鼠血清TNF-α,IL-6及胃组织IRAK-4 mRNA表达的影响  

Effects of Jianpi Wenwei Powder on Serum TNF-α,IL-6 and IRAK-4 mRNA Expression in Gastric Tissues of Spleen-deficiency Gastritis Rats

作　　者：冯果[1,2,3] 武静[1] 陈继婷[1] 刘文[1,3] 何新[2] 何前松[1] 吴增光[1] 

机构地区：[1]贵阳中医学院,贵阳550025 [2]天津中医药大学,天津300193 [3]国家苗药工程技术研究中心,贵阳550025

出　　处：《中国实验方剂学杂志》2016年第14期145-150,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省科学技术厅贵阳中医学院科学技术联合基金项目(黔科合中药字[2011]LKZ7025号)

摘　　要：目的:探讨健脾温胃散对脾气虚胃炎大鼠血清肿瘤坏死因子-α(TNF-α),白细胞介素-6(IL-6)及胃组织白细胞介素-1受体相关激酶-4(IRAK-4)mRNA表达的影响,为中药方剂治疗脾气虚胃炎提供实验依据。方法:Wistar大鼠60只,随机分正常组,胃炎模型组,阳性药物组(丽珠得乐胶囊,0.016 g·kg^(-1)·d^(-1))及健脾温胃散高、中、低剂量组(0.324,0.259,0.194g·kg^(-1)·d^(-1)),除正常组外均建立大鼠脾气虚胃炎动物模型,测量各组大鼠的肛温变化,各组大鼠ig相应药物30 d治疗后,采用放射免疫法测定其血清中IL-6,TNF-α的含量;苏木素-伊红(HE)染色观察胃组织病理学情况;逆转录-聚合酶链式反应(RT-PCR)方法观察健脾温胃散对胃炎大鼠模型胃黏膜IRAK-4 mRNA表达的影响。结果:与正常组比较,模型组大鼠肛温变化显著降低,大鼠血清IL-6,TNF-α含量显著升高,大鼠胃组织IRAK-4 mRNA表达显著升高(P<0.01),大鼠胃黏膜的病理损伤较明显;与模型组比较,健脾温胃散高、中、低剂量组能明显改善模型大鼠的证候特征及肛温变化,改善胃黏膜的病理形态,能显著降低胃炎模型大鼠血清IL-6,TNF-α含量(P<0.01),下调胃组织IRAK-4 mRNA表达(P<0.01)。结论:健脾温胃散能使模型大鼠的肛温恢复正常,改善胃黏膜的病理形态,降低脾气虚胃炎模型大鼠血清IL-6,TNF-α含量,抑制IRAK-4表达,可能是其治疗胃炎的作用机制之一。Objective： To investigate the effect of Jianpi Wenwei powder on the expression of serum tumour necrosis factor-α （TNF-α）, interleukin-6 （IL-6） and interleukin-1 receptor-associated kinase 4 （IRAK- 4） mRNA in the gastric mucosa of spleen-deficiency rats. Method： Totally 60 Wistar rats were randomly divided into normal control group, gastritis model group, positive drug control group （Colloidal Bismuth Subcitrate capsules, 0. 016 g·kg-1·d-1） and Jianpi Wenwei powder high dose group, middle dose group, low dose group （0. 324, 0.259, 0. 194 g·kg-1·d-1）. Except for the normal control group, all of the remaining groups were included in the spleen deficiency animal model of gastritis. The changes of anus temperature were measured in rats. At 30 d after the treatment for each group, IL-6, TNF-α in serum were determined by radioimmunoassay, histopathological changes in gastric tissues were observed by htoxylin eosin （HE） staining; reverse transcription polymerase chain reaction （RT-PCR） method was used to observe the effect of Jianpi Wenwei powder on the expression of IRAK-4 mRNA in gastric mucosa of chronic gastritis model rats. Result： Compared with the normal group, the model group showed significant decrease in the rectal temperature changes, and increases in serum IL- 6, TNF-α and IRAK-4 mRNA expression in gastric tissues （P 〈 0.01 ）, with obvious pathological injury at gastric mucosa of rats; Compared with the model group, Jianpi Wenwei Powder can significantly alleviate the syndrome characteristics and rectal temperature changes in model rats, release the pathological morphology of gastric mucosa, significantly decrease serum IL-6, TNF-α in rats of gastritis model （P 〈 0.01 ） , down-regulate the IRAK-4 mRNA expression in the gastric mucosa （P 〈 0.01 ）. Conclusion： Jianpi Wenwei powder can recover the anus temperature of model rats to normal, alleviate the pathological morphology of gastric mucosa, reduce the content of serum IL-6 and TNF-α in the rat

关 键 词：健脾温胃散 脾气虚胃炎 白细胞介素-6 肿瘤坏死因子-α 白细胞介素-1受体相关激酶-4 

分 类 号：R285.5[医药卫生—中药学]