杜仲多糖对链脲佐菌素致糖尿病小鼠的作用  

Effects of Eucommiae Cortex Polysaccharide on Streptozotocin-induced Diabetic Mice

作　　者：苏卓 郭诚 梁韬[2] 

机构地区：[1]广西梧州市中医院,广西梧州543000 [2]广西医科大学附属口腔医院,南宁530021

出　　处：《中国实验方剂学杂志》2016年第14期159-162,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西高校科学技术研究项目(LX2014085)

摘　　要：目的:研究杜仲多糖对链脲佐菌素(STZ)致糖尿病小鼠的作用及影响,并探讨相关机制。方法:取50只SPF级昆明小鼠按剂量120 mg·kg^(-1)ip STZ,ip前禁食12 h。3 d后,测量小鼠空腹血糖(FBG),小鼠FBG≥11.1 mol·L^(-1)表明糖尿病小鼠模型造模成功。将造模成功的小鼠随机分成5组,分别为模型组,二甲双胍组(32 mg·kg^(-1)),杜仲多糖高、中、低剂量组(500,250,125 mg·kg^(-1)),每组10只。另设正常组小鼠10只。连续ig给药14 d,每天1次。正常组和模型组ig给予等体积的生理盐水。末次给药前禁食12 h,给药1 h后拔眼球取血,离心取血清待测。测定14 d小鼠FBG,放射免疫测定法测定小鼠血清中空腹胰岛素(FNS)含量,计算胰岛素敏感指数(ISI),试剂盒测定血清中甘油三酯(TG),总胆固醇(TC),高密度脂蛋白胆固醇(HDL-C),低密度脂蛋白胆固醇(LDL-C)的含量;酶联免疫吸附测定(ELISA)测定血清中肿瘤坏死因子-α(TNF-α)和白细胞介素-8(IL-8)和IL-6;蛋白质免疫印迹(Western blot)法测定胰腺组织中转录因子-κB(NF-κB),Toll样受体4(TLR4)的蛋白含量。结果:与正常组比较,模型组小鼠FBG和血脂TG,TC,LDL-C水平明显升高,HDL-C水平,FNS和ISI含量明显降低,血清中TNF-α,IL-8和IL-6含量明显升高,胰腺组织中TLR4,NF-κB蛋白的表达明显升高(P<0.01);与模型组比较,杜仲多糖各剂量组明显降低小鼠FBG和血脂TG,TC,LDL-C水平,明显升高HDL-C水平,FNS和ISI含量,明显降低血清中TNF-α,IL-8和IL-6含量,明显降低胰腺组织中TLR4,NF-κB蛋白的表达(P<0.05,P<0.01)。结论:杜仲多糖具有降血糖和降血脂作用,其作用机制可能与提高糖尿病小鼠机体抗氧化能力、降低炎症因子血清中的含量,以及降低胰腺中TLR4,NF-κB蛋白含量相关。Objective： To investigate the effects of Eucommiae Cortex polysaccharide on streptozotocininduced diabetic mice. Method： Fifty SPF Kunming mice were intraperitoneally injected with 120 mg·kg-1 streptozotocin （STZ） after being fasted over 12 hours. After 3 days, the fasting blood glucose （FBG） of mice were measured to be ≥ 11.1 mol. L-1, indicating the successful modeling. The diabetic mice were randomly divided into 5 groups, with 10 mice per group： model group, metformin group （32 mg·kg-1） , and high, medium and low-dose Eucommiae Cortex polysaccharide groups （500, 250, 125 mg·kg-1）. Another 10 healthy mice were considered as normal group. These mice were administrated intragastrically once a day for 14 days. Normal and model groups were given equal volume of saline. One hour after the last administration, the blood samples of 12- hour fasted mice were collected by drawing eyeballs and were centrifuged to separate serum for further measurement. The FBG were detected at the 14th day; the serum fasting insulin （FNS） was measured using radioimmunoassay and then the insulin sensitivity index （ISI） was calculated. Serum triglyceride （TG） , total cholesterol （TC）, high density lipoprotein cholesterol （HDL-C） and low density lipoprotein cholesterin （LDL-C） were analyzed using commercial kits. enzyme-linked immuno sorbent assay （ELISA） method was utilized to detect TNF-α, IL-8 and interleukin-6 （IL-6） levels. The protein levels of Toll-like receptor 4 （TLR4） and nuclear transcriptional factor-κB （NF-κB） in pancreas tissues were analyzed by Western blot. Result： Compared with the normal group, the model group showed significant increases in FBG, lipid TG, TC, LDL-C, serum TNF-α, IL-8 and lL-6 contents, and protein expressions of TLR4 and NF-κB in pancreas, and significant decreases in HDL-C, FNS and ISI （P 〈 0. 01 ）; Compared with the model group, Eucommiae Cortex polysaccharide groups showed significant decreases in FBG, lipid TG, TC, LDL-C,

关 键 词：杜仲多糖 链脲佐菌素 糖尿病 血脂 血糖 炎性症因子 TOLL样受体4 核转录因子-ΚB 

分 类 号：R285.5[医药卫生—中药学]