青蒿素哌喹片对恒河猴经口给药急性毒性实验  

Acute Toxicity Test of Artemisinin and Piperaquine Tablets in Rhesus Monkeys by Oral Administration

作　　者：郭起岳[1] 宋健平[1,2] 茹丽[1] 袁征[1] 刘婵[1] 关业枝[1] 徐勤[1,2] 王琪[1,2] 郭嘉雯[1] 

机构地区：[1]广州中医药大学科技产业园,广州510445 [2]广州中医药大学,广州510006

出　　处：《中国实验方剂学杂志》2016年第14期167-170,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省科技计划项目(2011B010500008;2012B010300003)

摘　　要：目的:考察青蒿素哌喹片(ATQ)对恒河猴单次经口给药可能产生的的毒性反应,评价其安全性。方法:采用近似致死量法进行实验。对恒河猴单次经口给予青蒿素哌喹片,给药剂量为168,377,849,1 910,4 296 mg·kg^(-1),每个剂量1只动物,观察14 d内恒河猴的毒性反应和死亡情况,求得ATQ对恒河猴的近似致死剂量范围,称量体重、摄食量,检测血液学及血液生化学,对所有恒河猴解剖并进行大体病理观察。结果:所有恒河猴14 d内未出现死亡。4 296,1 910 mg·kg^(-1)剂量ATQ可使恒河猴出现呕吐及摄食减少,其中4 296 mg·kg^(-1)剂量组恒河猴体重减轻。结论:ATQ对恒河猴单次经口给药的近似致死剂量大于4 296 mg·kg^(-1)(相当于临床剂量的257倍)。Objective： To investigate the possible toxic reactions of Artemisinin and Piperaquine tablets in rhesus monkeys by oral administration, and evaluate its safety. Method： Approximate lethal dose method was used for the experiment. Artemisinin and Piperaquine tablet was given to 5 rhesus monkeys at the doses of 168, 377, 849, 1 910 , 4 296 mg·kg-1 respectively. Animals were observed with a period of 14 days for the toxic reactions and death. Their body weight, food intake, haematology and blood biochemistry indexes were measured, and gross pathological observation was conducted after anatomy in all rhesus monkeys, in order to calculate the range of approximate lethal dose. Result： No death appeared during 14 days observation. Vomiting and food intake reduction appeared in doses of 4 296, 1 910 mg·kg-1, and decrease in body weight was observed in monkey at the dose of 4 296 mg·kg-1. Conclusion： Approximate lethal dose of Artemisinin and Piperaquine tablets is greater than 4 296 mg·kg-1 （equivalent to 257 times of clinical dose） in rhesus monkeys by oral administration.

关 键 词：青蒿素哌喹片 急性毒性 恒河猴 血液学 血液生化学 

分 类 号：R285.5[医药卫生—中药学]