山东近海习见鱼类DNA条形码及其电子芯片分析  

DNA barcoding and electronic microarray for common fish species in Shandong coastal waters

作　　者：柳淑芳[1,2] 李献儒 杜腾飞[1,5] 庄志猛[1,3] 

机构地区：[1]中国水产科学研究院黄海水产研究所,山东青岛266071 [2]青岛海洋科学与技术国家实验室,海洋渔业科学与食物产出过程功能实验室,山东青岛266200 [3]青岛海洋科学与技术国家实验室,海洋生物学与生物技术功能实验室,山东青岛266200 [4]大连海洋大学水产与生命学院,辽宁大连116023 [5]上海海洋大学水产与生命学院,上海201306

出　　处：《中国水产科学》2016年第4期777-790,共14页Journal of Fishery Sciences of China

基　　金：国家自然科学基金项目(31172411);山东省科技发展计划项目(2012GHY11531);泰山学者建设工程专项经费项目

摘　　要：从GenBank下载到13目50科73属77种山东近海鱼类的线粒体细胞色素c氧化酶I(CO I)序列,通过分析其遗传距离及系统进化,并基于CO I序列筛选物种特异性探针来分析DNA芯片技术在进行物种鉴定时的可行性。结果表明,在CO I基因DNA条形码的分析中,77种鱼类的种间遗传距离(平均0.117)明显大于种内遗传距离(平均0.0034),且每个物种的不同个体在进化树上都能聚在一起,提示DNA条形码能全部区分77个物种;根据CO I基因设计的用于芯片的特异性探针中,77个物种最终有64个可以筛选出物种特异性探针,占总物种数的83.1%,本研究旨在为山东近海鱼类物种鉴定提供了技术支持。Marine biodiversity is an important indicator of ecosystem health and the material basis for the devel-opment and utilization of marine biological resources. Shandong coastal waters are the main breeding grounds for Bohai and Yellow Sea fisheries resources. These waters provide a wealth of biological resources and a superior fisheries habitat for China, Japan, South Korea, and North Korea. However, the Shandong offshore fisheries re-sources have been trending downward in recent years, and species abundance and diversity have decreased. Therefore, identifying and classifying the fish species in these coastal waters are urgently required to effectively protect and use these resources. In this study, 229 DNA sequences of the cytochrome oxidase subunit I （COI） gene from 77 common marine fish species in 13 orders, 50 families, and 73 genera from offshore of Shandong were analyzed to test the efficacy of species identification using a DNA barcode microarray. The results showed that interspecific genetic distance was larger than intraspecific distance. All 77 sequences formed species units in a neighbor-joining dendrogram, indicating that DNA barcodes can be used to identify these 77 species. Sixty-four specific probes were screened to identify the corresponding species among the 77 species based on theCOI genes and accounted for 83.1%. Thus, the DNA barcode microarray provided technical support and a new way to identify fish species in the coastal waters offshore of Shandong.

关 键 词：山东近海 CO I DNA条形码 DNA芯片 

分 类 号：S917[农业科学—水产科学]