跑台运动对肥胖大鼠海马内GLUT4和CD36等的影响  

Effects of Treadmill Exercise on GLUT4,CD36 and Related Proteins in the Hippocampus of Obese Rats

作　　者：张云丽[1,2] 娄淑杰[1] 蔡明[1] 李静静[1] 辛磊[1] 

机构地区：[1]上海体育学院运动科学学院,上海200438 [2]聊城大学体育学院,山东聊城252059

出　　处：《体育科学》2016年第7期56-62,共7页China Sport Science

基　　金：国家自然科学基金项目(81572241);上海市人类运动能力开发与保障重点实验室(上海体育学院)基金项目(11DZ2261100)

摘　　要：目的:探讨8周有氧耐力运动对肥胖大鼠海马内葡萄糖转运体4(Glucose transporter4,GLUT4)和脂肪酸转位酶(fatty acid translocase,TAT/CD36)及其相关指标的影响。方法:7周龄SD雄性大鼠随机分为普通饲料组(C)和高脂饲料组(H),分别以普通饲料和高脂饲料饲养8周后,C组大鼠随机等分为普通饲料安静组(NC)和普通饲料运动组(NE)。选取H组中体质量超过C组大鼠平均体质量加1.4倍标准差的大鼠20只,随机等分为肥胖安静组(OC)和肥胖运动组(OE)。NC组和OC组大鼠不运动,NE组和OE组大鼠经1周适应性运动后进行8周中等强度的跑台运动。末次运动结束后24h,禁食过夜,每组大鼠中随机选取6只,10%水合氯醛麻醉,冰上分离海马组织,Western blotting法检测海马内GLUT4、CD36、PI3K、p-PI3K、AMPKα、p-AMPKα、ACC和p-ACC的表达。结果:肥胖大鼠海马内CD36的蛋白表达显著增加,但GLUT4、PI3K、AMPKα和ACC的蛋白表达以及PI3K、AMPKα和ACC的磷酸化水平均无显著变化。跑台运动对肥胖大鼠海马内CD36、GLUT4、PI3K、AMPKα和ACC的蛋白表达以及PI3K、AMPKα和ACC的磷酸化水平均无显著性影响。结论:实验证实大鼠海马内存在CD36的蛋白表达,并且首次发现高脂膳食诱导的肥胖大鼠出现海马内CD36的蛋白表达显著增加,但跑台运动未能对肥胖大鼠海马内GLUT4和CD36及其相关指标产生显著性影响。Objective：To investigate the impact of 8-week aerobic endurance exercise training on glucose transporter 4（GLUT4）,fatty acid translocase（CD36）and related indexes in the hippocampus of obese rats.Methods：7-week-old male SD rats were randomly divided into normal diet group（C）and high fat diet group（H）.The rats were maintained for 8weeks on either normal diet or high fat diet.And then,the rats in group C were randomly and equally divided into normal diet sedentary group（NC）and normal diet exercise group（NE）.Twenty rats in group H,whose body weight values were above the mean value plus 1.4times the standard deviations of the body weights of rats in group C,were picked out and then randomly divided into obese sedentary group（OC）and obese exercise group（OE）.Rats in group NC and group OC were without exercise training.Rats in group NE and group OE were performed an8-week moderate intensity treadmill exercise training after being adapted to the treadmill for a week.The rats were fasted overnight 24 hours after the last exercise training,and then 6rats were randomly selected in each group,anaesthetized with 10% chloral hydrate.Hippocampal tissues were isolated in ice bath.The expressions of GLUT4,CD36,PI3 K,p-PI3 K,AMPKα,p-AMPKα,ACC and p-ACC were detected by Western blotting.Results：The protein expression of CD36 in the hippocampus of obese rats was significantly increased,but the protein expressions of GLUT4,PI3 K,AMPKαand ACC as well as the phosphorylation levels of PI3 K,AMPKαand ACC were not significantly changed.The treadmill exercise training did not show significant effects on the protein expressions of CD36,GLUT4,PI3 K,AMPKαand ACC as well as the phosphorylation levels of PI3 K,AMPKαand ACC in the hippocampus of obese rats.Conclusion：The results demonstrated that CD36 protein was expressed in rat hippocampus,and this study found for the first time that CD36 protein expression increased significantly in the hippocampus of high fat diet-induced obese rats,while the trea

关 键 词：大鼠 海马 运动 高脂膳食 肥胖 葡萄糖转运体4 脂肪酸转位酶 

分 类 号：G804.2[文化科学—运动人体科学]