青藏高原木孜塔格冰川、玉珠峰冰川及扎当冰川可培养细菌的生理特征  

The physiological characteristics of culturable bacteria in Muztag、Yuzhufeng and Zadang Glaciers on Tibetan Plateau,China

作　　者：邢婷婷[1] 刘勇勤[2,3] 王宁练[2,4] 沈亮[1] 顾政权 郭泌汐 

机构地区：[1]中国科学院青藏高原研究所高寒生态学与生物多样性重点实验室,北京100101 [2]中国科学院青藏高原地球科学卓越中心,北京100101 [3]中国科学院青藏高原研究所青藏高原环境变化与地表过程重点实验室,北京100101 [4]中国科学院寒区旱区环境与工程研究所冰冻圈科学国家重点实验室,甘肃兰州730000

出　　处：《冰川冻土》2016年第2期528-538,共11页Journal of Glaciology and Geocryology

基　　金：国家自然科学基金项目(41425004;41190084;41371084)资助

摘　　要：为了解冰川微生物生长特点,分析了青藏高原木孜塔格冰川、玉珠峰冰川和扎当冰川可培养细菌在不同温度,及木孜塔格冰川可培养细菌在不同盐度和p H下的生长特性.木孜塔格冰川52%的可培养细菌不耐盐,只能在0%盐度下生长,38%的细菌可以在0%~4%/6%盐度培养基中生长,其余细菌可以在0%~1%/2%的盐度培养基中生长,且62%的细菌具有较广的p H值生长范围（p H 5~9）;另外,38%的细菌只能在弱酸性（5%）或者只能在弱碱性（33%）培养基中生长.3个冰川可培养细菌生长温度范围均为0~35℃,木孜塔格冰川最适生长温度≤20℃的细菌占其细菌总数的86%,而玉珠峰冰川和扎当冰川最适生长温度≤20℃的细菌则分别占其细菌总数的69%和53%.不同冰川具有不同最适生长温度的细菌的比例不同,同一冰川不同深度相同属类的细菌有相近的生长温度特征、耐盐度和耐酸碱特征.In order to reveal the characteristics of glacier-microorganism,we assayed the culturable bacteria iso-lated from Muztag Glacier,Yuzhufeng Glacier and Zadang Glacier at different temperatures. Besides,culturable bacteria isolated from Muztag Glacier was observed at different salinity and pH. Salinity endurance of 52% of Muztag Glacier culturable bacteria was 0%,and 38%bacteria could grow in the medium of NaCl density with 0%~4%/6%,while the other bacterial salinity tolerance range was 0%~1%/2%. Muztag Glacier culturable bac-teria had wide range pH tolerance（5~9）occurring 62%,while bacteria only grew in weak acid（5%）or weak alkaline（33%）medium accounting for 38%. Growth-temperature range of bacteria in these three glaciers was 0~ 35 ℃. The Muztag bacterial optimum temperature which was below 20 ℃（including 20 ℃）accounted for 86%,while Yuzhufeng Glacier and Zadang Glacier were 69%and 53%,respectively. The percent of different op-timum temperatures in different glaciers were different. Culturable bacteria belonged to one genus isolated from same glacier had similar growth-temperature range,salinity and pH tolerance in different depths.

关 键 词：冰川微生物 温度 盐度 pH 

分 类 号：Q935[生物学—微生物学]