红花注射液关节内注射预防膝关节术后粘连的实验研究  

The prophylactic effects of safflor injection of salvia miltiorrhiza on jiont adhesions in rabbit knee joints

作　　者：阮锋[1] 袁冰[1] 刘平[1] 李贺伟[1] 韦卓[1] 

机构地区：[1]华中科技大学同济医学院附属医院梨园医院骨科,湖北武汉430077

出　　处：《生物骨科材料与临床研究》2016年第3期7-9,共3页Orthopaedic Biomechanics Materials and Clinical Study

基　　金：华中科技大学自主创新研究基金(NO.2014QN071)

摘　　要：目的探索红花注射液关节内注射对预防膝关节术后粘连的效果,为膝关节术后粘连的临床治疗提供参考。方法选择成年健康的雄性日本大耳白兔24只,体重3.5-4.0kg,随机分成3组：A组（透明质酸钠对照组）、B（红花注射液实验组）组和C组（盐水空白对照组）,每组8只,所有动物均行右侧膝关节滑膜切除术,术后右下肢管型石膏伸直位固定4周,分组予生理盐水、透明质酸钠、红花注射液关节内重复注射每周1次,每组每只共4次,对每组动物进行膝关节视觉计分、生物力学分析、组织学观察及膝关节滑膜平均胶原含量分析。结果生物力学实验表明A、B组大于C组（PP〈P〈0.01）,A、B组之间无显著差异（pP〉P〈0.05）;膝关节视觉计分A、B组均于小C组（PP〈P〈0.01）,A、B组之间无显著差异（pP〉P〈0.05）;组织学观察A、B组均小于C组（PP〈P〈0.01）,A、B组之间无显著差异（pP〉P〈0.05）;膝关节滑膜平均胶原含量测定A、B组小于C组（PP〈P〈0.01）,B组小于A组（PP〈P〈0.01）。结论红花注射液关节内注射可很好的防止膝关节术后粘连的发生,效果与透明质酸钠无显著差异。Objective To explore the intra-articular injections of safflower injection effect on knee joint of postoperative adhesion prevention, provide reference for clinicaltreatment of knee joint of postoperative adhesion. Methods Adult healthy male Japanese big ear rabbit 24,3.5 to 4.0 kg weight, randomly divided into 3 groups： group A（sodium hyaluronate group） and B（safflower injection group） group and C group（saline blank control group）, each group of eight, all animals are on the right side of the knee joint synovial resection, postoperative 4 weeks right leg tube type gypsum was fixed, the group to the physiological saline, sodium hyaluronate, safflower injection intra-articular repeated injection once A week, each group A total of 4 times each knee visual score on each group of animals, biomechanics analysis,histologic observation and knee joint synovial average collagen content analysis. Results Biomechanical experiments showed A and group B than in group C（ PP〈P〈0.01）, there was no significant difference between A and B group（ PP〈P〈0.05）;Knee visual points A and B were in small C group（ PP〈P〈0.01）, there were no significant difference between A and B group（ PP〈P〈0.05）; Histological observation of A and B group were less than group C（P〉 P〈0.01）, there was no significant difference between A and B group（ PP〈P〈0.05）; Knee joint synovial average collagen content determination of A and B is less than C group（P〉 P〈0.01）, group B is less than group A（P〉 P〈0.01）. Conclusion Intra-articular injections of safflower injection can well prevent knee postoperative adhesion, there was no significant difference effect with sodium hyaluronate.

关 键 词：膝关节粘连 红花注射液 透明质酸 

分 类 号：R687.4[医药卫生—骨科学]