5-HT_(2C)受体对帕金森病模型大鼠外侧缰核谷氨酸能神经元电活动的影响  

Effects of 5-HT_(2C) receptors on the firing activity of the glutamate neurons in the lateral habenular nucleus of the hemiparkinsonian rats

作　　者：韩玲娜[1] 王春雷[2] 刘小静[3] 

机构地区：[1]长治医学院生理学教研室,山西长治046000 [2]长治医学院生物医学工程系,山西长治046000 [3]西安交通大学第一附属医院,陕西西安710061

出　　处：《陕西师范大学学报（自然科学版）》2016年第4期70-76,共7页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：陕西省自然科学基金(2014JQ168)

摘　　要：为探讨帕金森病(PD)模型大鼠外侧缰核(LHb)中谷氨酸能神经元的电活动变化及其对5-羟色胺2C(5-HT_(2C))受体刺激的反应,阐明LHb和5-HT_(2C)受体在PD神经生物学机制中发挥的作用,通过6-羟多巴胺(6-OHDA)损毁单侧黑质致密部(substantia nigra pars compacta,SNc)建立PD模型,采用细胞外记录的方法,观察假手术组与单侧SNc损毁组大鼠LHb中谷氨酸能神经元的电活动。结果显示:与假手术组大鼠相比,损毁组大鼠LHb中谷氨酸能神经元的电活动增强,表现为爆发式放电活动增强(P<0.05);LHb中局部注射5-HT_(2C)受体激动剂Ro60-0175后,两组大鼠的神经元放电频率虽均升高(P<0.05),但损毁组大鼠LHb中谷氨酸能神经元放电频率升高持续时间显著长于假手术组,且给药前后神经元的放电形式更趋向于爆发式活动(P<0.05),变异系数明显升高(P<0.05)。结果提示单侧SNc损毁使LHb中谷氨酸能神经元爆发式电活动增强,可能与PD相关抑郁行为发生有关,5-HT_(2C)受体参与了对LHb中谷氨酸能神经元电活动的调节。To study the changes in firing activity of the glutamate neurons and the responses of the neurons to 5-hydroxytryptamine 2C（5-HT（2C））receptors stimulation in the lateral habenualr nucleus（LHb）in rats with unilateral 6-hydroxydopamine（6-OHDA）lesions of the substantia nigra pars compacta（SNc）,and clarify the role of the LHb and 5-HT（2C） receptors in the mechanism of PD,the firing activities of glutamate neurons in the LHb of sham-operated and the6-OHDA-lesioned rats were assessed by electrophysiological study.The results showed that unilateral lesions of the SNc in rats led to a more bursting-firing activity of LHb glutamate neurons（P〈0.05）.Intra-LHb injection of Ro60-0175 as agonist of 5-HT（2C） receptors resulted in increase in the firing rate of LHb glutamate neurons（P〈0.05）and the percentage of burst-firing neurons（P〈0.05）in sham-operated and the 6-OHDA-lesioned rats.Compared to sham-operated rats,the duration of the excitatory effect produced by Ro60-0175 was markedly longer in thelesioned rats.These findings indicate that the changes in firing activity of glutamate neurons may be involved in the parkinsonian-related depression.And 5-HT（2C） receptors are involved in the regulation of the firing activity of glutamate neurons in the LHb.

关 键 词：帕金森病 外侧缰核 谷氨酸能神经元 5-HT2C受体 

分 类 号：R338.8[医药卫生—人体生理学]