氯化锂复合磷酸钙骨水泥可促进大鼠胫骨骨缺损修复  

Effect of a lithium-doped calcium phosphate cement in promoting tibial bone defect repair in rats

作　　者：李理[1] 覃永保 马刚[1] 李兵[1] 

机构地区：[1]广西医科大学第四附属医院//柳州市工人医院骨科,广西柳州545005

出　　处：《南方医科大学学报》2016年第6期824-828,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(81260273);广西自然科学基金(2013GXNSFAA019269)~~

摘　　要：目的观察磷酸钙骨水泥(CPC)与氯化锂复合磷酸钙骨水泥(Li/CPC)对SD大鼠骨折愈合的影响。方法取20只6月龄雌性SD大鼠,设立Li/CPC实验组(n=10)及CPC对照组(n=10),分别行双侧胫骨骨缺损填充术,术后1、2月每组分别处死5只大鼠,取胫骨标本行显微CT(Micro-CT)检测及组织学切片HE染色,进行比较分析。结果 Micro-CT扫描显示,Li/CPC组骨缺损修复效果比CPC组好,Li/CPC组材料周围新骨体积分数(BV/TV)较CPC组高,骨小梁分离度(Tb.Sp)较小,差异有统计学意义(P<0.05);组织学HE染色显示,Li/CPC组纤维骨痂及骨样骨痂出现较早,且数量较多,骨愈合质量明显高于CPC组。结论 Li/CPC具有较好的骨传导性能,能明显促进骨缺损修复。Objective To evaluate the effects a lithium chloride-doped calcium phosphate cement（Li/CPC） on promoting tibial defect repair in rats. Methods Twenty 6- month- old female SD rats randomized into Li/CPC（n=10） and CPC control（n=10）groups. Surgery was performed to create bone defects at the bilateral tibia, which were filled with either of the cement. Five rats from each group were sacrificed at 1 and 2 months after the surgery for micro- CT examination and HE staining of the tibia. Results Micro- CT showed better repair of bone defects in Li/CPC group, which had a significantly higher new bone volume fraction（BV/TV） and a lower trabecular separation/spacing（Tb.Sp） than the control group（P〈0.05）. HE staining showed an earlier appearance of fiber and osteoid callus in Li/CPC group than CPC control group. The number and quality of bone healing was significantly higher in Li/CPC group than in CPC group. Conclusion Li/CPC possessed better osteoinductivity and can significantly promote bone defect repair in rats.

关 键 词：磷酸钙骨水泥 氯化锂 显微CT HE染色 骨缺损修复 

分 类 号：R318.08[医药卫生—生物医学工程] R687[医药卫生—基础医学]