蒙药复方森登-4特征图谱及其中6个指标性成分的含量测定  

Specific chromatogram of Mongolian medicine compound Sendeng-4 and simultaneous determination of its six index components

作　　者：白埔 萨础拉[2] 董玉[1] 

机构地区：[1]内蒙古医科大学药学院,内蒙古010059 [2]内蒙古医科大学蒙医药学院

出　　处：《北京中医药大学学报》2016年第6期489-493,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.81160550);内蒙古自治区自然科学基金项目(No.2013JQ03);内蒙古医科大学"英才培育"项目

摘　　要：目的建立蒙药复方森登-4特征图谱及同时测定其中没食子酸、京尼平-1-β-D-龙胆二糖苷、栀子苷、（2R,3R）-双氢杨梅素、芦丁、杨梅素6个成分含量的方法。方法采用高效液相色谱‘HPLC’法。蒙药复方森登-4特征图谱色谱系统：用Extend-C18色谱柱（250 mm×4.6 mm,5μm）,以乙腈（A）-0.6%磷酸水溶液（B）为流动相,流速为1 m L/min,检测波长为254 nm,柱温为25℃,进样量为10μL。蒙药复方森登-4中6个成分含量测定色谱系统：用Extend-C18（250 mm×4.6mm,5μm）色谱柱,以乙腈（A）-0.6%磷酸水溶液（B）为流动相,流速为0.5 m L/min,检测波长为254 nm和292 nm,柱温为27℃,进样量为10μL。结果蒙药复方森登-4特征图谱共检出90多个峰,与标准品HPLC图谱比对,共指认出10个峰,其中6个峰可以含量测定。经后续HPLC含量测定,6个成分的含量均在线性范围内,并与其各自峰面积积分值呈良好的线性关系（均r=0.999 9）;平均回收率在98.29%~103.75%之间,RSD为1.44%~2.97%。结论本法准确、简便、重复性好,可作为蒙药复方森登-4的质量控制方法。Objective To establish HPLC specific chromatogram of Sendeng-4,a Mongolian medicine compound,and to simultaneously determine six index components—gallic acid,genipin-1-β-D-gentiobio-side,geniposide,（2R,3R）-dihydromyricetin,rutin,andmyricetin.Methods HPLCspecificchro-matogram was obtained under the following system：Extend-C18 column （250 mm×4.6 mm,5 μm）, mobile phase of acetonitrile （A）and 0.6 % phosphoric acid aqueous solution （B）,gradient elution, flow rate at 1 mL/min,the column temperature at 25 ℃,detection wavelength at 254 nm,and the injec-tion volume 10 μL.HPLC system for determination was listed as the following：Extend-C18 column （250 mm ＆#215;4.6 mm,5 μm）,mobile phase of acetonitrile （A）and 0.6%phosphoric acid aqueous solution （B）,gradient elution,flow rate at 0.5 mL/min,the column temperature at 27 ℃,the detection wave-lengthsat254nmand292nm,andtheinjectionvolume10μL.Results Morethan90peakswerede-tected on HPLC specific chromatogram,from which ten were identified by comparing with standard HPLC chromatogram and six were measured.After determination,the content and the peak area of each compo-nent had a good linear relationship,with correlation coefficients 0.999 9,average recovery of 98.29%~103.75%andRSD1.44%~2.97%.Conclusion The method established is accurate,simpleandre-peatable,and can be used to control the quality of Mongolian medicine compound Sendeng-4.

关 键 词：蒙药复方森登-4 特征图谱 没食子酸 京尼平-1-β-D-龙胆二糖苷 栀子苷 (2R 3R)-双氢杨梅素 芦丁 杨梅素 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]