8个新疆扁桃S-基因多态性与生物信息学研究  

Studies on Polymorphism and Bioinformatics of 8 Xinjiang Almonds Self- incompatibility Gene( S-gene)

作　　者：郭春苗[1] 杨波[1] 吐迪.麦麦提 李宁[1] 唐亚萍[1] 龚鹏[1] 王继勋[1] 

机构地区：[1]新疆农业科学院园艺作物研究所/农业部新疆地区果树科学观测试验站,乌鲁木齐830091 [2]新疆莎车县林业局,新疆莎车844700

出　　处：《新疆农业科学》2016年第6期1067-1073,共7页Xinjiang Agricultural Sciences

基　　金：自治区公益性科研院所基本科研业务经费资助项目"基于S-基因型鉴定的新疆本地扁桃种质资源鉴定(KY2015072)";国家自然科学基金项目"新疆‘纸皮’扁桃幼果生理脱落与蔗糖代谢关系的生理与分子机制研究"~~

摘　　要：【目的】自交不亲和基因(S-基因)在果树及授粉树选配和自交亲和性品种选育等方面具有重要意义,是影响扁桃繁殖性状的重要候选基因,对8个新疆扁桃S-基因序列、基因结构、基因多态性进行研究。【方法】采用Blast、Prot Param和Signal P等生物信息学软件,对8个新疆扁桃S-基因的理化性质、多态性、系统发生树和蛋白结构等进行分析。【结果】新疆扁桃S氨基酸存在信号肽,不是分泌性蛋白,具有跨膜结构,推测S氨基酸可能定位于细胞膜中发挥功能;通过进化树分析,分为两大类群;二级结构由无规则卷曲、α螺旋和延伸链等结构元件组成。【结论】8个新疆扁桃S-基因的理化性质、多态性、系统发生树和蛋白结构等生物信息学分析结果将为S-基因的酶学特性及自交不亲和基因分子机理研究提供理论参考。【Objective】Self incompatibility gene（ S- gene） is an important candidate gene affecting reproductive traits of almond. It has important significance in the selection of self compatible varieties of fruit tree and the selection of pollination tree. This paper is preliminary studies on S- gene sequence,gene structure,gene polymorphism of 8 Xinjiang almond.【Method】This experiment used the Blast,Protparam and Signalp bioinformatics software to analyze the physical and chemical properties,polymorphism,phylogenetic tree and protein structure of 8 Xinjiang almond S- gene.【Result】The S amino acid of Xinjiang almond contained signal peptide and had a membrane structure,but it wasn＇t a secretory protein. It has been assumed that S amino acid may be located in the cell membrane to play a function. Through phylogenetic tree analysis,they were divided into two groups; The secondary structure was composed of irregular coil,alpha helix,extended chain,etc.【Conclusion】The analysis results of S- gene sequence,gene structure,gene polymorphism of 8Xinjiang almond will provide a theoretical reference for the enzymatic properties of S- gene and the molecular mechanism of self incompatibility gene.

关 键 词：扁桃 S-基因 多态性 生物信息学分析 

分 类 号：S662.9[农业科学—果树学]