盐胁迫对苜蓿愈伤组织细胞形态结构的影响  

Effects of Salt Stress on Morphological Structure of Alfalfa Callus Cells

作　　者：李珍[1] 秦艳筠 龚无缺 黄唯子 杨静慧[1] 刘艳军[1] 

机构地区：[1]天津农学院园艺园林学院,天津300384

出　　处：《天津农学院学报》2016年第2期21-24,共4页Journal of Tianjin Agricultural University

基　　金：天津市重大科技专项"优质早熟浆果新品种引种和耐盐碱育种及设施栽培"(12ZCDZNC04800);天津市科技特派员项目"耐盐耐旱彩叶植物品种筛选和耐盐机理研究"(14JCTPJC00530);天津市科技成果转化及产业化推进计划项目"优质彩叶北美海棠新品种种苗的规模化繁育"(14ZXNZNC004)

摘　　要：为了解盐胁迫对苜蓿细胞形态结构的影响,更好地进行耐盐诱变育种,本研究以青睐苜蓿为试材诱导分化了耐盐愈伤组织,并对其愈伤组织细胞的形态和结构进行了比较。结果表明:盐胁迫使愈伤组织细胞面积减小,仅为对照的1/4,对照愈伤组织细胞半径是耐盐愈伤组织细胞的2.22倍,液泡半径是耐盐细胞的2.93倍;盐胁迫对细胞核和叶绿体大小影响不大,但使愈伤组织细胞变短,细胞形状更接近于球形,细胞核位于细胞中间,单位面积中的叶绿体数量增加,并使愈伤组织外观呈黄绿色,细胞排列紧密、均匀,硬度增加。In order to learn about the effect of salt stress on morphological structure of alfalfa cells for better breeding salt-tolerant varieties, the morphology structure of two kinds of salt-tolerant alfalfa callus induced by Qinglai alalfa was studied. The results showed that callus cell area under salt stress reduced and was only a quarter of the CK. Besides, the radius of CK callus cells was 2.22 times that of the salt-tolerant cells and the vacuole radius of CK callus cells was 2.93 times that of the salt-tolerant cells; Although the influence of salt stress on the size of cell nuclear and chloroplast was insignificant, the callus cells became shorter and was closer to the spherical shape. The cell nucleus was in the center of the cell, and the number of chloroplasts per unit volume was increased, so that the color of the salt-tolerant callus was green-yellow and the cells were uniform, packed tightly and had high hardness.

关 键 词：盐胁迫 苜蓿 愈伤组织 细胞 形态结构 

分 类 号：S541.1[农业科学—作物学]