蓬子菜含药血清对H_2O_2诱导的人脐静脉内皮细胞损伤模型细胞增殖与凋亡的影响  

Effect of Galium Verum L Medicated Serum on the Proliferation and Apoptosis of HUVECs Injured by Hydrogen Dioxide

作　　者：薛凤[1] 苑海刚[2] 赵钢[2] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040

出　　处：《中医药导报》2016年第15期21-23,共3页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：黑龙江省研究生创新科研基金(2015007)

摘　　要：目的:观察蓬子菜含药血清对H2O2诱导的人脐静脉内皮细胞(HUVECs)损伤模型细胞增殖与凋亡的影响,从分子生物学水平阐明蓬子菜保护血管内皮细胞的部分机制。方法:用不同浓度(0%,2%,5%,10%)的蓬子菜含药血清及通塞脉片含药血清与体外培养的经H2O2诱导的人脐静脉内皮细胞损伤模型共同孵育,以CCK-8法检测细胞的增殖活性,流式细胞仪检测细胞凋亡及细胞内活性氧(ROS)含量。结果:与对照组比较,蓬子菜含药血清能够明显减轻H2O2对人脐静脉内皮细胞的增殖抑制作用,促进HUVECs的增殖,并与浓度呈正相关;能显著降低细胞内活性氧的含量,减少H2O2诱导的HUVECs凋亡;且优于正常血清组及通塞脉片血清组(P<0.05)。结论:蓬子菜能促进内皮细胞增殖,降低细胞内活性氧的含量,减少细胞凋亡,从而阻止ASO的发生,达到预防和治疗ASO的作用。Objective： To observe the effects of Galium verumL medicated serum on proliferation and apopto- sis of human umbilical vein endothelial cells （HUVECs） injured by hydrogen dioxide and illuminate some mech- anism of Galium verumL protecting HUVECs on the molecular biology level. Methods： Cultured HUVECs which injured by hydrogen dioxide were incubated with different concentrations of Galium verumL medicated serum and Tongsaimai tablets medicated serum（0%, 2%, 5%, 10%）. CCK-8 was used to measure cell proliferation of HUVECs. The flow cytometry assay was used to measure cell apoptosis and the expression levels of ROS. Re- sults： Compared to the control group, the proliferation of HUVEC was facilitated and the apoptosis was signifi- cantly inhibited by co-incubating with Galium verumL medicated serum, and there was a positive correlation be- tween the different concentrations of the medicated serums. The curative effect of Galium verumL medicated serum group is significantly superior to the Tongsaimai tablets medicated serum group （P〈0.05）. Conclusions： Galium verumL can facilitate the proliferation and inhibit the apoptosis of HUVECs, and effectively prevent the onset of ASO.

关 键 词：蓬子菜 内皮细胞 增殖 细胞凋亡 细胞内活性氧 

分 类 号：R285.5[医药卫生—中药学]