紫苏叶总黄酮提取物对过氧化氢所致人肾小管上皮细胞HK-2的氧化损伤保护作用  

Protective effects of perilla frutescens britt var. japonica total flavonoid on hydrogen peroxide (H_2O_2)-induced oxidative stress in HK-2 cells

作　　者：马丽娜[1] 黄纯绚 莫晓晖[3] 

机构地区：[1]浙江大学医学院附属儿童医院,浙江杭州310003 [2]广西壮族自治区妇幼保健院,广西南宁530021 [3]玉林市中西医结合骨科医院,广西玉林537000

出　　处：《华夏医学》2016年第3期14-17,共4页Acta Medicinae Sinica

摘　　要：目的:探讨紫苏叶总黄酮(PLFE)对过氧化氢(H_2O_2)所致人肾小管上皮细胞氧化损伤的保护作用。方法:将HK-2细胞与H_2O_2和不同浓度的PLFE(10,25,50,100μg/ml)共培养24 h,用MTT法观察PLFE对H_2O_2致损伤HK-2细胞活性的影响;硫代巴比妥酸法(TBARS)测定细胞内脂质过氧化产物丙二醛(MDA)含量,检测细胞内源性过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化酶(GSH-px)的含量,观察分析PLFE对H_2O_2致损伤HK-2细胞的保护作用。结果:H_2O_2(损伤组)对HK-2细胞有明显的细胞毒性,PLFECF对H_2O_2所致HK-2细胞毒性损伤有保护作用。PLFE组细胞生存率与对照组比较,差异有统计学意义(P<0.05)。PLFE组对H_2O_2所致HK-2细胞的CAT、SOD和GSH-px的酶活性高于损伤组,差异有统计学意义(P<0.05)。结论:紫苏叶黄酮提取物对H_2O_2导致的HK-2细胞氧化损伤具有保护作用。Objective： To investigate the protective effects of perilla frutescens britt var. japonica total flavonoid （PLFE） against hydrogen peroxide （H2O2）-induced oxidative damage in human renal proximal tubule HK-2 cells. Methods： HK-2 cells were co-incubated with H2O2 and different concentrations of PLFE （10, 25, 50, 100μg/ml ） together for 24 h. HK-2 cell viability was determined by MTF assay. The levels of lipid peroxidation were measured by the TBA reactive substance （TBARS） assay. The endogenousantioxidant enzymes including catalase （CAT）, superoxide dismutase （SOD） and glutathione peroxidase （GSH） levels were determined by commercial assay kits according to the manufacturer＇s instructions. Results： It was found that H2O2 could induce the oxidative damage on HK-2 cells and decrease the content of antioxidant enzyme. Cell survival rate of PLFE,compared with that of the control group was of statistical significances （P〈0.05）. PLFE could increase the content of antioxidant enzymes of CAT, SOD and GSH-px more than those of the control group, which were of statistical significances （P〈0.05）. Conclusion： PLFE shows an activity to protect HK-2 cells against H2O2-induced oxidative damage

关 键 词：脑胶质瘤 

分 类 号：R692.6[医药卫生—泌尿科学]