ThERF1 from Tamarix hispida confers decreased tolerance to oxidative and drought stresses and is regulated by a WRKY protein  

作　　者：Liuqiang Wang Chao Wang Liping Qin Ping Hu Yucheng Wang 

机构地区：[1]State Key Laboratory of Tree Genetics and Breeding(Northeast Forestry University),Harbin 150040, China [2]Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 8300ll, China

出　　处：《Journal of Forestry Research》2016年第4期767-772,共6页林业研究（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.31270703);the Special Fund for Outstanding Talented Person of Harbin City(2012RFXXN023);100 Talents Program of The Chinese Academy of Sciences

摘　　要：The ethylene-responsive factor family is one of the largest families of plant-specific transcription factors that are involved in plant development and stress responses. Previously, we demonstrated that the gene Th ERF1,encoding a novel ethylene-responsive factor from Tamarix hispida, negatively modulates abiotic stress tolerance. In the present study, Arabidopsis plants overexpressing Th ERF1 had decreased oxidative tolerance and increased transpirational water loss rate compared with wild-type plants, leading to sensitivity to abiotic stress. Real-time RT-PCR showed that the upstream regulator of Th ERF1,Th WRKY2, is involved in responses to different abiotic stresses. Furthermore, both Th WRKY2 and Th ERF1 shared similar expression patterns in the stems and leaves of T.hispida when exposed to salinity, drought and abscisic acid. Chromatin immunoprecipitation assays further confirmed that Th WRKY2 can directly bind to the promoter of Th ERF1 and regulate its expression. This study revealed the regulatory mechanism of Th ERF1 expression in response to abiotic stresses in T. hispida.The ethylene-responsive factor family is one of the largest families of plant-specific transcription factors that are involved in plant development and stress responses. Previously, we demonstrated that the gene Th ERF1,encoding a novel ethylene-responsive factor from Tamarix hispida, negatively modulates abiotic stress tolerance. In the present study, Arabidopsis plants overexpressing Th ERF1 had decreased oxidative tolerance and increased transpirational water loss rate compared with wild-type plants, leading to sensitivity to abiotic stress. Real-time RT-PCR showed that the upstream regulator of Th ERF1,Th WRKY2, is involved in responses to different abiotic stresses. Furthermore, both Th WRKY2 and Th ERF1 shared similar expression patterns in the stems and leaves of T.hispida when exposed to salinity, drought and abscisic acid. Chromatin immunoprecipitation assays further confirmed that Th WRKY2 can directly bind to the promoter of Th ERF1 and regulate its expression. This study revealed the regulatory mechanism of Th ERF1 expression in response to abiotic stresses in T. hispida.

关 键 词：WRKY drought Tamarix abiotic responsive transgenic encoding Arabidopsis chlorophyll promoter 

分 类 号：S793.5[农业科学—林木遗传育种]