空间培养箱中实时观察GFP标记开花基因表达方法与技术  

Method and Technology of Monitoring the Expression of Flowering Gene Marked with Green Fluorescent Protein in Space Culture Chamber

作　　者：王丽华[1] 谢俊燕[1] 张岳[1] 郑慧琼[1] 

机构地区：[1]中国科学院上海生命科学研究院植物生理生态所,上海200032

出　　处：《空间科学学报》2016年第4期562-565,共4页Chinese Journal of Space Science

基　　金：国家载人航天天宫二号工程项目;中国科学院战略性先导科技项目(XDA04020202);国家重点基础研究发展计划项目(2011CB710902)共同资助

摘　　要：绿色荧光蛋白(Green Fluorescent Protein,GFP)已被广泛用作一种强有力的生物发光报告蛋白.但是,其在空间植物培养箱中应用尚存很大困难.本文提出一种在空间植物培养箱中利用LED作为激发光源,对拟南芥中开花基因启动子控制下GFP基因表达的观察与分析方法,为在空间生物学实验中研究基因表达模式提供了新的方法和技术.Green fluorescent Protein（GFP） has been widely used as a powerful bio-luminescence reporter molecule for monitoring gene expression on ground.However,it is still difficult to use GFP as a tool in the space culture chamber for life studies.In this study,we present a technical method,which use the LED as excitation light source,for monitoring and analysis of GFP gene expression of the flowering locus T gene in Arabidopsis thaliana that is controlled by the promoter.The method in this study provides a new technical reference for the gene expression studies in space.

关 键 词：空间培养箱 绿色荧光蛋白质 开花基因 拟南芥 

分 类 号：V524.1[航空宇航科学与技术—人机与环境工程]