HPLC测定金沙排石冲剂中芍药苷含量  

Determination of albiflorin in the Jinsha-Paishi infusion by HPLC

作　　者：刘川玉[1] 唐建红[2] 邓俊刚[1] 

机构地区：[1]桂林医学院附属医院药学部,541001 [2]桂林医学院第二附属医院药学部,541199

出　　处：《国际中医中药杂志》2016年第7期633-636,共4页International Journal of Traditional Chinese Medicine

基　　金：广西中医药科技专项课题（GZZJ13-18）

摘　　要：目的：建立HPLC测定金沙排石冲剂中芍药苷含量的方法，并测定不同批次金沙排石冲剂中芍药苷含量。方法色谱柱为Zorbax C18（250 mm×4.6 mm，5μm），流动相为甲醇-水（25∶75，V/V），流速为1.0 ml/min，检测波长为230 nm，柱温为室温，进样量为20μl，测定3个批次金沙排石冲剂的芍药苷含量。结果芍药苷的进样量在2.5～200.0μg范围内与峰面积线性关系良好（r＝0.9992）；精密度、稳定性和重复性试验的RSD值均＜2%；平均回收率为98.05%，RSD为1.65%。芍药苷含量分别为0.080、0.083、0.077 mg/g。结论本方法准确灵敏、稳定可靠、重复性好，可用于金沙排石冲剂中芍药苷的质量控制，不同批次金沙排石冲剂中芍药苷含量差别不大。Objective To establish HPLC assaying methods for the determination of albiflorin in the Jinsha-Paishi infusion, and to determine the content of albiflorin of different batches ofJinsha-Paishiinfusion. Methods The chromatographic conditions were with the column of C18 （250 mm ＆#215; 4.6 mm, 5μm）, mobile phase of methyl alcohol-aqua （25：75,V/V）, flow rate of 1.0 ml/min, and determined at wave length of 230 nm in room temperature. A total of 3 different batches of albiflorin were determined.Results In the range of 2.5-200μg, the distribution of paeoniflorin was linear （r=0.999 8）, and theRSD of precision, stability and repeatability were less than 2%. The average recovery was 98.05%, andRSD was 1.65%. The contents of albiflorin in each batch were 0.080, 0.083, 0.077 mg/g.Conclusion The assaying method of HPLC is convenient, accurate and reliable to determinate the content of albiflrin in theJinsha-Paishi infusion. And the results showed that there was no difference of content of albiflorin among the different batches ofJinsha-Paishi infusion.

关 键 词：高效液相色谱 金沙排石冲剂 芍药苷 含量测定 

分 类 号：R286.0[医药卫生—中药学]