降糖消渴颗粒对糖尿病大鼠骨骼肌AMPK信号通路的影响  

Effects of Jiangtang Xiaoke Granule on AMPK Signal Pathway of Muscular Tissues of Diabetic Rats

作　　者：赵丹丹[1] 莫芳芳[1] 穆倩倩 张毅[2] 安宏[2] 田甜[2] 吴瑞[3] 高思华[1] 

机构地区：[1]北京中医药大学糖尿病研究中心,北京100029 [2]北京中医药大学基础医学院,北京100029 [3]中国中医科学院广安门医院南区,北京100000

出　　处：《辽宁中医杂志》2016年第7期1525-1527,I0005,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：重大新药创制子课题(2012ZX09103201-005);国家国际科技合作项目(2011DFA30920);国家自然科学基金项目(NSFC81503540)

摘　　要：目的:探讨肝脾肾同治的组方降糖消渴颗粒对糖尿病(DM)大鼠血糖及骨骼肌AMPK信号通路的影响。方法:SD大鼠高脂饲料喂养4周后结合腹腔注射链脲佐菌素(STZ)30 mg·kg^(-1),建立2型糖尿病大鼠模型,将成模的大鼠按血糖随机分为模型组、阳性对照组(吡格列酮片1.5 mg/kg BW)、降糖消渴颗粒组(9 g生药/kg BW),并设正常对照组。干预前及干预2周、4周后,分别检测大鼠空腹血糖(FBS),实验结束后实时荧光定量PCR法检测大鼠肌肉组织AMPKα、葡萄糖转运子4(GLUT4)、胰岛素受体底物1(IRS-1)mRNA表达,Western Blot法检测AMPKα、p-AMPKα、GLUT4、IRS-1蛋白表达。结果:降糖消渴颗粒组大鼠空腹血糖水平均明显低于模型组大鼠(P<0.05),且该效果存在时-效关系。降糖消渴颗粒能上调DM大鼠骨骼肌组织AMPKαmRNA及蛋白表达,且p-AMPK蛋白量亦明显增加(P<0.01),同时提高DM大鼠骨骼肌组织GLUT4、IRS-1 mRNA及蛋白表达量(P<0.01)。结论:立足于肝脾肾三脏同治的中药复方降糖消渴颗粒能通过激活骨骼肌AMPK信号通路相关蛋白,达到改善DM大鼠糖代谢,提高骨骼肌胰岛素敏感性的作用。Objective： To explore the effect of Jiangtang Xiaoke Granule（ JTXKG）,a Chinese herbal medicine which was constructed based on treatment of diabetes by regulating spleen,liver and kidney together,on blood glucose level and AMPK signal pathway in experimental diabetic rats. Methods： Diabetic rats were induced by high- fat diet and low dose streptozotocin（ 30 mg·kg^（-1））. JTXKG 9 g/kg（ based on crude herb equivalent） and pioglitazone 1. 5 mg/kg（ as a positive control for comparison）were orally administrated to DM rats for 4 weeks. The normal and drug- untreated DM rats were administrated with the same volume of vehicle. The fasting blood glucose（ FBG） was measured and the mRNA levels of AMPKα,GLUT4 and IRS- 1 and the protein expression levels of AMPKα,p- AMPKα,GLUT4 and IRS- 1 in muscle tissues were detected. Results： JTXKG reduced the FBG levels in a dose- dependent manner. JTXKG could significantly up- regulate the mRNA levels and protein expressions of AMPK- α,GLUT4 and IRS- 1,as well as the p- AMPK α protein level in skeletal muscle of the diabetic rats. Conclusion：JTXKG could regulate the glucose metabolism and increase the insulin sensitivity by inducing the AMPK signal pathway in the muscle tissue of diabetic rats.

关 键 词：降糖消渴颗粒 2型糖尿病 骨骼肌 AMPK信号通路 

分 类 号：R285.5[医药卫生—中药学]