棉花纤维中苯丙烷类结构单体的检测方法  

A Method for Detecting Phenylpropanoid Monomers in Cotton Fiber

作　　者：胡文冉[1,2] 范玲 孙涛[3] 谢丽霞[2] 田晓莉[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193 [2]新疆农业科学院核技术生物技术研究所/新疆农作物生物技术重点实验室/新疆农业科学院棉花分子机理与分子育种实验室,新疆乌鲁木齐830091 [3]新疆农业科学院农业质量标准与检测技术研究所,新疆乌鲁木齐830091

出　　处：《棉花学报》2016年第4期407-412,共6页Cotton Science

基　　金：新疆维吾尔自治区创新人才项目(2014721025)

摘　　要：苯丙烷类化合物存在于棉花纤维中,并且与纤维品质的形成有关。为正确评价棉花纤维中存在的苯丙烷类结构单体,首先采用巯基乙酸法提取出棉花纤维中的苯丙烷类化合物,然后用衍生化后的还原裂解(DFRC,Derivatization followed by reductive cleavage)法结合气相色谱(GC,Gas chromatograph)对棉花纤维中的苯丙烷类单体进行测定。结果表明,利用该方法检测到棉花纤维苯丙烷类单体主要由愈创木基结构单体(G￣木质素)和紫丁香基结构单体(S￣木质素)组成。本研究为进一步揭示棉花纤维中苯丙烷类化合物的合成奠定了基础。In the plant cell wall, the phenylpropane monomer affects the properties of the polymers derived from phenylpropanoid compounds. Recent studies have found that the phenylpropane metabolism（the second largest metabolic pathway after the glucose metabolism） exists in developing cotton fibers. Phenylpropanoid compounds present in cotton fiber are related to the formation of quality fiber. Because the phenylpropanoid compounds contents of cotton fibers are very low, and mature cotton fibers have dead, hollow, soft, dried cell walls that are difficult to triturate with conventional grinding techniques, the existing methods used to directly analyze the phenylpropanes monomer are unsuitable for use in cotton fibers. In this experiment, to evaluate phenylpropanoid monomers in cotton fibers, phenylpropanoid compounds were extracted from cotton fiber using thioglycolic acid, then using the derivatization followed by the reductive cleavage method and gas chromatograph analysis, the phenylpropanoid monomers of cotton fiber were determined. The results showed that guaiacyl monomer（G-lignin） and syringyl monomer（S-lignin） are present in cotton fibers. This study will lay a basis for further analysis of phenylpropanoid compounds in cotton fiber.

关 键 词：棉花纤维 苯丙烷类结构单体 巯基乙酸法 衍生化后的还原裂解法 

分 类 号：TS107.2[轻工技术与工程—纺织工程]