蚌埠市2015年麻疹疑似病例实验室检测结果分析  

Analysis of laboratory test results of suspected measles cases in Bengbu city in 2015

作　　者：付广林[1] 董娟[1] 刘国洲[1] 孙莉[1] 

机构地区：[1]蚌埠市疾病预防控制中心,安徽蚌埠233000

出　　处：《安徽预防医学杂志》2016年第3期180-182,共3页Anhui Journal of Preventive Medicine

摘　　要：目的分析2015年蚌埠市麻疹疑似病例实验室检测结果,了解麻疹流行状况。方法采集2015年共784份疑似麻疹病例血清标本,采用酶联免疫捕获法检测血清标本中麻疹Ig M抗体,采用荧光定量PCR法检测咽拭子标本中麻疹病毒核酸。结果检测血清标本784份,麻疹Ig M抗体阳性261份,阳性检出率为33.29%;检测咽拭子标本128份,麻疹病毒核酸阳性67份,阳性检出率为52.34%。结论血清学检测和核酸检测互为补充,结合运用能够提高麻疹病原学诊断的检出率。Objective To analyze the results of laboratory test of suspected measles cases in Bengbu city in 2015,and understand the prevalence of measles. Methods The Ig M antibody of measles in serum was detected by ELISA,and the measles virus nucleic acid was detected by fluorescence quantitative PCR method. Results There were 261 Ig M positive samples detected in total 784 serum samples,and the positive rate was 33. 29%. There were 67 measles virus nucleic acid positive samples detected in total 128 throat swab samples,and the positive rate was 52. 34%. Conclusion Complementary application of serological detection and nucleic acid detection could improve the detection rate in the diagnosis of measles.

关 键 词：麻疹病毒 ELISA 荧光定量PCR 麻疹抗体 麻疹病毒核酸 

分 类 号：R511.1[医药卫生—内科学]