靶向ELAM-1的磁共振分子探针的构建与表征  

Construction and Characterization of MR Molecular Probe Targeted Endothelial Cell Adhesion Molecule-1

作　　者：刘树英[1] 古冬连 赖少侣[1] 黄秒[1] 康巍[1] 金观桥[1] 苏丹柯[1] 

机构地区：[1]广西医科大学附属肿瘤医院放射科,广西南宁530021

出　　处：《中国CT和MRI杂志》2016年第8期130-133,共4页Chinese Journal of CT and MRI

基　　金：国家自然科学基金资助项目(NO.81260334和81460452)

摘　　要：目的探讨用超顺磁性氧化铁(USPIO)螯合唾液酸化酶X(s LeX)形成靶向内皮细胞粘附分子-1(ELAM-1)的特异性磁共振成像的分子探针的制备方法,并研究其物理化学特性。方法利用物理沉积方法合成USPIO纳米颗粒,通过疏水作用,合成较好水溶性的PEG-USPIO,其表面?COOH充分活化,常温下与s LeX充分孵育,超滤离心和去离子水洗涤,形成磁共振分子探针USPIO-PEG-s LeX,测定其表征。结果透射电镜测定PEG-SPIO平均粒径为(10±2.6)nm,分散性较好,大小适宜;动态光散射测定偶联前后其平均水动力尺寸分别为(34.06±9.95)nm,(53.35±16.99)nm;偶联前后的PEG化磁性纳米颗粒的Zeta电位分别为(11.6±3.96)m V,(-12.6±5.33)m V。结论化学交联法可成功制备磁共振分子探针USPIO-PEG-s LeX,该分子探针具有良好表征,有望满足体内、外实验特异性结合ELAM-1的要求。Objective To investigate the methods of preparing a specific molecular probe for magnetic resonance imaging targeting endothelial cell adhesion molecule-1 （ESLAM-1） with ultrasmall superparamagnetic particles of iron oxide（USPIO） chelating sialyl-Lewis X （sLe^X）, and research its physicochemical properties. Methods Using physical deposition method to synthesis USPIO nanoparticles. By hydrophobic interactions, preferable water-soluble PEG-SPIO was synthesized and the surface -COOH was sufficiently activated,then incubated with sufficient sLe^X at room temperature, purified by centrifugal ultrafiltration and washed with deionized water. The magnetic resonance molecular probe USPIO-PEG-sLex was prepared and characterize it. Results TEM results revealed that the PEG-SPIO had a size of （10± 2.6）nm with good dispersibility and suitable size. DLS study showed that before and after coupling, the hydrodynamic mean diameter were （34.06±9.95）nm and （53.35 ±16.99） nm, respectively. Zeta potential study showed that before and after coupling the potential of PEG conjugated magnetic nanoparticles potential were （11.6 ±3.96） mV and （-12.6± 5.33） mV,respectively. Conclusion The method of chemical conjugation can be successfully prepared MR1 molecular probe USPIO-PEG- sLe^X, which has a well-characterized molecular probe, and it is expected to meet vivo experiments specifically binds to ELAM-1 requirements.

关 键 词：磁共振分子成像 超顺磁性氧化铁 内皮细胞粘附分子-1 

分 类 号：R445.2[医药卫生—影像医学与核医学]