Acclimation and Characterization of a Pseudomonas Strain for Improved Phosphorus Removal  

作　　者：秦华星 田晴 李方 朱艳彬 杨波 

机构地区：[1]College of Environmental Science and Engineering,Donghua University

出　　处：《Journal of Donghua University(English Edition)》2016年第3期403-410,共8页东华大学学报（英文版）

基　　金：National Natural Science Foundation of China(No.51478099);Scientific Research Foundation for Returned Overseas Chinese Scholars,China(No.SEM-11W11329);Natural Science Foundation of Shanghai,China(No.16ZR1402000)

摘　　要：A Pseudomonas strain(named as P.PAO-1) with phosphorous removal function was isolated and characterized.A new method of two-stage cultivation was applied to the strain to induce the synthesis of intracellular polyhydroxylbutyrate(PHB) in the cells.In the first stage,bacterial cells were enriched under a high carbon source condition;in the second stage,the bacteria cells were cultivated under the nutrient-imbalanced conditions with the carbon source-regulated medium.As a result,the PHB content of strain P.PAO-1 reached 22.8% compared with the normal 7.41% in the non-acclimated strain.This change had led to the rising of P uptake from 1 to 25 mg·L^(-1).When added the strain P.PAO-1into the activated sludge(with the addition proportion of 1:1),the ratio of biological phosphorus removal increased by 14.9%under the normal alternating anaerobic/aerobic conditions with low-carbon consumption.The results demonstrated that the isolated pure culture strain P.PAO-1 belonged to the functional group of poly-P accumulating microorganism.When cultured by the two-stage cultivation method,the initial accumulation of PHB in the strain cells could be achieved and the phosphorous removal capacity of strain P.PAO-1 could be induced subsequently.When applied to the wastewater,strain P.PAO-1 performed phosphorus removal from the wastewater with or without the addition of activated sludge.A Pseudomonas strain（named as P.PAO-1） with phosphorous removal function was isolated and characterized.A new method of two-stage cultivation was applied to the strain to induce the synthesis of intracellular polyhydroxylbutyrate（PHB） in the cells.In the first stage,bacterial cells were enriched under a high carbon source condition;in the second stage,the bacteria cells were cultivated under the nutrient-imbalanced conditions with the carbon source-regulated medium.As a result,the PHB content of strain P.PAO-1 reached 22.8% compared with the normal 7.41% in the non-acclimated strain.This change had led to the rising of P uptake from 1 to 25 mg·L^（-1）.When added the strain P.PAO-1into the activated sludge（with the addition proportion of 1：1）,the ratio of biological phosphorus removal increased by 14.9%under the normal alternating anaerobic/aerobic conditions with low-carbon consumption.The results demonstrated that the isolated pure culture strain P.PAO-1 belonged to the functional group of poly-P accumulating microorganism.When cultured by the two-stage cultivation method,the initial accumulation of PHB in the strain cells could be achieved and the phosphorous removal capacity of strain P.PAO-1 could be induced subsequently.When applied to the wastewater,strain P.PAO-1 performed phosphorus removal from the wastewater with or without the addition of activated sludge.

关 键 词：phosphorus accumulating organism(PAO) carbon sourceregulated medium enrichment ami acclimation Pseudomonas Putida 

分 类 号：X703.1[环境科学与工程—环境工程]