孟鲁司特对哮喘大鼠肺组织嗜铬蛋白A表达的影响  

A study on the influence of montelukast on chromogranin A protein and mRNA expressions at lung tissue of asthmatic rat

作　　者：吕青青[1] 金小红[1] 李绍波[1] 童夏生[2] 张维溪[3] 罗冬娇[4] 

机构地区：[1]温州医科大学附属台州医院,浙江临海317000 [2]台州市中西医结合医院 [3]温州医科大学附属育英儿童医院 [4]杭州师范大学钱江学院

出　　处：《预防医学》2016年第8期781-784,共4页CHINA PREVENTIVE MEDICINE JOURNAL

基　　金：浙江省自然基金资助项目(LY13H080006);温岭市科技局基金资助项目(2012-2-109)

摘　　要：目的研究嗜铬蛋白A在哮喘发病中的表达及孟鲁司特的调控作用。方法将SD大鼠随机分为哮喘组、对照组和孟鲁司特组,采用卵白蛋白复制哮喘模型,分别采用免疫组化法和实时荧光定量PCR法检测大鼠肺组织嗜铬蛋白A及其mRNA表达情况,测定嗜铬蛋白A表达的光密度(OD)值强度,分析嗜铬蛋白A与其mRNA表达的相关性。结果哮喘组大鼠嗜铬蛋白A的表达水平(OD值0.34±0.05)高于对照组(OD值0.21±0.06)(P<0.01),嗜铬蛋白A mRNA表达水平(RQ值4.02±0.95)高于对照组(P<0.01);孟鲁司特组嗜铬蛋白A的表达水平(OD值0.28±0.04)低于哮喘组(P<0.05),嗜铬蛋白A mRNA表达水平(RQ值3.67±0.78)与哮喘组,差异无统计学意义(P>0.05)。大鼠肺组织嗜铬蛋白A与其mRNA的表达水平呈直线正相关(r=0.635,P<0.01)。结论嗜铬蛋白A可能参与哮喘的炎症机制,孟鲁司特能下调嗜铬蛋白A的表达。Objective To investigate the potential roles of chromogranin A in pathogenesis of asthmatic inflammation,and to assess the regulation of montelukast on chromogranin A expression.Methods The rat asthma model was established with ovalbumin,and they were allocated to three groups,named asthma group,control group and montelukast group.The expressions of chromogranin A protein and mRNA at lung tissue were detected by immunohistochemisty or real-time PCR methods,and positive expression intensity of chromogranin A protein was assayed by optical density.The correlation between chromogranin A protein and mRNA was also analyzed.Results The expression levels of chromogranin A protein in asthma group（0.34 ±0.05 optical density）was significantly higher than that in control group （0.21 ±0.06 optical density）（P〈0.01 ）.The expression levels of chromogranin A mRNA in asthma group （4.02 ±0.95 relative quantity value）was significantly higher than that in control group（P〈0.01 ）.The expression levels of chromogranin A protein in montelukast group（0.28 ±0.04 optical density）was dramatically lower than that in asthma group （0.34 ±0.05 optical density）（P〈0.05）,while there were no statistical significance of chromogranin A mRNA（3.67 ±0.78 relative quantity value）between those two groups（P〉0.05 ）.But levels of mRNA was positively correlated with protein of chromogranin A （r=0.635,P〈0.01）.Conclusion Expressions of chromogranin A protein and mRNA at lung tissue were increased in asthmatic rats,and the results demonstrated that chromogranin A perhaps participated in the pathogenesis of asthma inflammation,but this function of chromogranin A protein could be down regulated by montelukast.

关 键 词：哮喘 嗜铬蛋白A 孟鲁司特 发病机制 

分 类 号：R974.3[医药卫生—药品]