miR-186通过靶定CEA抑制宫颈癌细胞转移  

miR-186 inhibits cervical cancer migration by targetting CEA

作　　者：孙艳[1] 齐政[1] 王德华[1] 张艳芳[1] 

机构地区：[1]天津医科大学总医院妇产科,天津300052

出　　处：《天津医科大学学报》2016年第4期288-290,294,共4页Journal of Tianjin Medical University

摘　　要：目的:验证miR-186通过靶定癌胚抗原(CEA)对宫颈癌细胞生物功能的影响。方法:利用表达谱芯片及荧光定量PCR验证miR-186在宫颈癌中的表达水平;通过Transwell及划痕试验阐述miR-186靶定CEA对宫颈癌细胞转移能力的影响;通过双荧光素酶报告基因方法验证miR-186对CEA的靶定关系。结果:转移性宫颈癌组织中miR-186的表达水平较非转移性宫颈癌组织中偏低;在宫颈癌细胞中上调miR-186的表达水平并靶定CEA能够抑制其转移能力;荧光报告载体试验证实CEA为miR-186的靶基因。结论:miR-186抑制宫颈癌的转移,CEA为miR-186的靶基因。Objective： To analyze the function of miR-186 in cervical cancer by targeting CEA. Methods： MicroRNA microarray and qRT-PCR were performed to analyze the expression level of miR-186, then transwell and wound healing assay were performed to analyze the effect of miR-186 over-expression on cervical cell lines. In addition, luciferase reporter assay was used to locate the target gene of miR- 186. Results： The expression of miR-186 in matastasis cervical cancer was lower than that in non-matastasis cervical cancer tissues. Ectopic expression of miR-186 inhibited cervical cell migration. The result of Luciferase reporter assay showed that CEA was directly regulated by miR-186. Conclusion： MiR-186 is lowly expressed in matastasis cervical cancer and inhibited cervical cancer cell migration through targeting CEA.

关 键 词：miR-186 宫颈癌 癌胚抗原 转移 

分 类 号：Q7[生物学—分子生物学]