FGF-2上调Survivin蛋白的表达拮抗心肌细胞凋亡  

FGF-2 Inhibits Cardiomyocytes Apoptosis Induced by Hypoxia/reoxygenation via Upregulating the Expression of Survivin in H9c2 cells

作　　者：李国华[1] 刘米华 彭娟[1] 肖卫晋 屈顺林[1] 唐之晗 郭芳[1] 彭利军[1,3] 任重[1] 危当恒[1] 刘录山[1] 王佐[1] 姜志胜[1] 

机构地区：[1]南华大学心血管疾病研究所动脉硬化学湖南省重点实验室,湖南衡阳421001 [2]南华大学附属南华医院检验科 [3]南华大学儿科学院湖南省儿童医院科教科

出　　处：《中南医学科学杂志》2016年第4期379-383,389,共6页Medical Science Journal of Central South China

基　　金：国家自然科学基金项目(81470435;81541005);湖南省卫生厅科研计划课题(B2011-041)

摘　　要：目的探讨成纤维细胞生长因子-2(FGF-2)拮抗心肌细胞凋亡的作用机制。方法以H9c2大鼠心肌细胞作为实验对象,用无血清培养基同步化培养24小时,心肌细胞随机分为正常组(Control)、缺氧/复氧(H/R)组和H/R+FGF-2组。采用CCK-8比色法检测心肌细胞存活率,Hoechst染色和流式细胞术检测心肌细胞凋亡。采用Western blot检测生存素(Survivin)蛋白的表达。结果与正常组相比,H/R明显抑制心肌细胞活力,诱导心肌细胞凋亡(均P<0.01);与H/R组相比,FGF-2显著改善H/R心肌细胞活力,拮抗H/R心肌细胞凋亡,同时上调Survivin蛋白的表达(均P<0.05)。结论 FGF-2拮抗心肌细胞凋亡的作用与其上调Survivin蛋白表达有关。Objective To investigate the mechanism of FGF-2＇ s fighting against H9c2 cardiomyocytes apoptosis in- duced by Hypoxia/reoxygenation（H/R）. Methods H9c2 cardiomyocytes was employed as the experimental object,cells were incubated by free serum for 24 h for synchronous cell cycle, then, H9c2 cardiomyocytes were randomly divided into 3 groups：Normal group（Control） ,H/R group, H/R＋FGF-2 group.The viability of cardiomyocytes was measured by CCK-8. The apoptosis rate of cardiomyocytes was detected by Hoechst and Armexin-V/PI assays with flow cytometry. Survivin ex- pression were analyzed by western Blot. Results Compared to the Contrd group, H/R significantly decreased the viability of cardiomyocytes and induced cardiomyocytes apoptosis（P〈0.01 ）.Treatment with FGF-2 for H/R significantly increased the viability of cardiomyocytes subjected to H/R, suppressed cardiomyocytes apoptosis induced by H/R and up-regulated the expression of Survivin （P〈0.05）. Conclusion FGF-2 inhibits cardiomyocytes apoptosis induced by H/R in H9c2 cells, which the mechanism may be related to the up-regulation of Survivin expression.

关 键 词：成纤维细胞生长因子-2 细胞凋亡 生存素 

分 类 号：R541[医药卫生—心血管疾病]