超高效液相色谱与飞行时间质谱联用快速鉴别木芙蓉叶的化学成分  

Rapid Identification of Chemical Constituents of Folium hibisci Mutabilis by UHPLC-Q-TOF-MS/MS

作　　者：李军茂 何明珍[1,2] 欧阳辉[1,2] 谭婷[1,2] 李艳[1,2] 冯育林[1,2] 杨世林[1,2] 

机构地区：[1]江西中医药大学,南昌330004 [2]创新药物与高效节能降耗制药设备国家重点实验室,南昌330006

出　　处：《中国药学杂志》2016年第14期1162-1168,共7页Chinese Pharmaceutical Journal

基　　金：江西省青年科学家培养对象资助项目(20142BCB23022)

摘　　要：目的通过超高效液相色谱与飞行时间质谱联用(UHPLC-Q-TOF-MS/MS)技术对木芙蓉叶中的化学成分进行定性分析。方法采用UHPLC-Q-TOF-MS/MS,Welch C18色谱柱(2.1 mm×100 mm,1.7μm),以乙腈-0.1%甲酸溶液为流动相梯度洗脱;质谱采用电喷雾(ESI)离子源,在负离子模式下采集数据,通过保留时间、精确分子离子峰和二级质谱裂解碎片,对木芙蓉叶进行成分鉴定。结果初步鉴定了木芙蓉叶中73个化学成分,其中包括42个黄酮类,15个三萜类,11个有机酸类,3个香豆素类,其他类2个。结论该方法简单,快速,结果准确,为进一步阐明木芙蓉叶的药效物质研究奠定了基础。OBJECTIVE To qualitatively analyze the chemical constituents of Folium hibisci Mutabilis by high performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry （UHPLC-Q-TOF-MS/MS）. METHODS The sep- aration was performed on a UHPLC Welch C,s column （2. 1 mmx 100 ram, 1. 7 μm） , with acetonitrile-0. 1% formic acid as the mobile phase for gradient elution ; ESI ion source was used ; the data was collected in a negative ion mode. The chemical eomponents of Folium hibisci Mutabilis were identified by analyzing the retention time, exact relative molecular mass, and cleavage fragments of MS/ MS. RESULTS A total of 73 compounds were identified in Folium hibisci Mutabilis, including 42 flavonoids, 15 triterpenoids, 11 or- ganic acids,3 coamarins,and 2 other kinds of compounds by UPLC-Q-TOF-MS. CONCLUSION LC-MS/MS can identify the chemi- cal components of Fdium hibisci Mutabilis in a simple, which would provide a foundation for further exploration of the effective sub- stances of Folium hibisci Mutabilis.

关 键 词：木芙蓉叶 超高效液相色谱与飞行时间质谱联用 裂解规律 化学成分 

分 类 号：R284[医药卫生—中药学]