东革阿里中活性成分宽缨酮的提取及纯化工艺研究  

Extraction and Purification Process of the Active Ingredient Eurycomanone from Eurycoma longifolia Jack

作　　者：李红亮[1] 于秀颖[1] 马崇鸣 Kiew Lik-Voon Mohammad Rais Mustafa 李卫国 田景奎[1] 

机构地区：[1]浙江大学生物医学工程与仪器科学学院,杭州310027 [2]南京理工大学泰州科技学院,江苏泰州225300 [3]马来亚大学医学院药学系,马来西亚吉隆坡50603 [4]常熟求是科技有限公司,江苏常熟215500

出　　处：《中国药学杂志》2016年第14期1169-1175,共7页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(U1303122);江苏省科技支撑项目(BE2014654);常熟市科技支撑项目(CS201405)

摘　　要：目的优选东革阿里的提取纯化工艺。方法以宽缨酮Eurycomanone(EN)的转移率为考察指标,综合采用单因素实验和正交实验,通过对乙醇浓度、提取时间、溶剂用量、提取次数的考察,优选东革阿里合理的提取工艺;在静态吸附和解吸实验筛选树脂的基础上,结合单因素和正交实验优选东革阿里采用大孔树脂纯化的工艺。结果东革阿里的最佳提取工艺为:加20倍量水回流提取2次,第一次2h,第二次1h;最佳纯化工艺为:采用HPD100型大孔树脂,以70%饱吸附量上样,上样液质量浓度0.25 g生药·m L-1,上样流速3 BV·h-1,洗脱乙醇体积分数30%,洗脱流速3B V·h-1。结论优选出的工艺操作简单、稳定可行、重复性好,可推广于工业化生产应用。OBJECTIVE To optimize the extraction and purification process of eurycomanone （EN） from Eurycoma longifolia Jack. METHODS Using single factor test and orthogonal test to screen the best conditions of ethanol concentration, extraction time, amount of solvent and extraction times with the index of transfer rate of EN. Resin model was screened by static adsorption and desorp- tion test, purification process of EN was investigated by single factor test and orthogonal test. RESULTS The optimal condition of the extracting was 20-fold water, 2 times with the first 2 and 1 h for the second time. Optimal purification process of HPD100 macroporous resin was 70% amount of saturated adsorption sample, 0. 25 g · mL- 1 of sample liquid concentration, 3 BV · h - 1 of sample flow rate, e- luting using the 30% ethanol, 3 BV · h-l of elution velocity. CONCLUSION The optimized process is simple, stable and repeat- able, which is suitable for industrial production.

关 键 词：东革阿里 宽缨酮 正交实验 提取工艺 纯化工艺 

分 类 号：R282[医药卫生—中药学]