哮喘患者CD4^+ CD25^+调节性T细胞比例与炎症因子的相关性  

Correlation between the CD4^+ CD25^+ T Regulatory Cells Proportion and Serum Inflammatory Factors in Peripheral Blood of Asthmatic Patients

作　　者：高炜[1] 

机构地区：[1]北京市仁和医院,北京102600

出　　处：《贵阳医学院学报》2016年第7期851-854,共4页Journal of Guiyang Medical College

摘　　要：目的:探讨哮喘患者外周血CD4^+CD25^+调节性T细胞比例与血清中炎症因子的相关性。方法:110例哮喘患者均分为哮喘急性发作组和哮喘缓解组,55例健康体检者作为对照组;用流式细胞仪测定哮喘患者外周血中CD4^+CD25^+调节性T细胞的比例,酶联免疫法(ELISA)检测被检者血清中白细胞介素18(IL-18)、IL-4及转化生长因子β(TGF-β)含量;用Spearman统计方法分析哮喘患者外周血中CD4^+CD25^+调节性T细胞的比例与血清中IL-18、IL-4和TGF-β含量的相关性。结果:与对照组比较,哮喘缓解组、哮喘急性发作组患者全血细胞中CD4^+CD25^+T细胞比例降低,血清中IL-18、IL-4及TGF-β含量升高(P<0.05);与哮喘缓解组比较,哮喘急性发作组外周血中CD4^+CD25^+T细胞的比例降低而血清中IL-18、IL-4及TGF-β含量升高(P<0.05);Spearman相关分析结果显示,哮喘患者外周血CD4^+CD25^+T细胞比例均与血清IL-18、IL-4和TGF-β含量呈显著负相关关系(r=-0.712、-0.629、-0.593,P=0.006、0.008、0.005)。结论:外周血CD4^+CD25^+T细胞的比例与哮喘的发生和发展密切相关,其机制可能与IL-18、IL-4和TGF-β升高有关。[ Abstract]Objective：To investigate the relationship between the CD4 ^＋CD25 ^＋T regulatory cells proportion and serum inflammatory factors in peripheral blood of asthmatic patients. Methods：110 ca-ses of patients with asthma were selected as the research subjects,and divided into asthma acute attack group（ n=55 ）and asthma remission group（ n=55 ）. Another 55 healthy people were selected as con-trol group. Enzyme linked immunosorbent assay（ELISA）was used to detect the content of IL-18,IL-4 and TGF-β,and flow cytometry was used to detect the proportion of CD4 ^＋CD25 ^＋T regulatory cells in peripheral venous blood. Spearman statistical method was adopted to analyze the correlation between the CD4 ^＋CD25 ^＋T regulatory cells proportion and serum inflammatory factors in peripheral blood of asthmatic patients. Results：Compared with control group,CD4 ^＋CD25 ^＋T regulatory cells proportion of blood cell in asthma acute attack group and asthma remission group was significantly decreased while the content of IL-18 ,IL-4 and TGF-β significantly was increased（ P﹤0. 05 ）. Compared with asthma remission group,in asthma acute attack group the CD4 ^＋CD25 ^＋T regulatory cells proportion of blood cell was significantly decreased while the content of IL-18 ,IL-4 and TGF-βsignificantly was increased （ P﹤0 . 05 ）. The correlation analysis showed that the contents of IL-18 ,IL-4 and TGF-β in asthma patients were negatively correlated with the proportion of CD4 ^＋CD25 ^＋T regulatory cells（ r = -0. 712 ,-0. 629 ,-0. 593 ,P =0. 006 ,0. 008 ,0. 005 ）. Conclusions：The proportion of CD4 ^＋D25 ^＋regulatory T cells is closely related to the occurrence and development of asthma,and its mech-anism may be related to content increase of IL-18 ,IL-4 and TGF-β.

关 键 词：哮喘 白细胞介素类 转化生长因子Β CD4^+CD25^+调节性T细胞 因素分析 统计学 TRANSFORMING growth factor β 

分 类 号：R562.25[医药卫生—呼吸系统]