亲和标签在重组蛋白纯化领域的研究进展  

Research progress of the affinity tag in the field of recombinant protein purification

作　　者：武文轩[1] 孙冬琳[1] 孙海明[1] 金焰[1] 

机构地区：[1]哈尔滨医科大学医学遗传学研究室,150081

出　　处：《国际遗传学杂志》2016年第3期138-143,共6页International Journal of Genetics

基　　金：教育部“创新团队发展计划”（IRT1230）;黑龙江省杰出青年基金（JC201215）

摘　　要：亲和纯化标签在重组蛋白纯化领域中发挥着重要作用，经过标准化流程即可得到高产量高纯度的靶蛋白。近年来，许多不同种类的纯化标签陆续被设计出来，其中包括短肽标签、表位标签、折叠蛋白结构域标签、非层析法标签，以及最近研究较多的复合标签。除了通过经典的蛋白酶去除靶蛋白所携带标签的方法之外，自我裂解去除标签的方法因其简便廉价等优点近年来逐渐受到重视。本文主要对重组蛋白纯化标签的研究进展进行综述。Engineered purification tags can very efficiently facilitate the purification of recombi -nant proteins , resulting in high yields and purities in a few standard steps .Over the years , many different purification tags have been developed, including short peptides , epitopes , folded protein domains , non-chromatographic tags and recently developed compound multifunctional tags with optimized capabilities .Al-though classic proteases are still used as a primary method to remove the tags from the target proteins , new self-cleaving methods are gaining more attention as a highly convenient alternative .In this review ,we dis-cuss some of these emerging trends , and examine their potential impacts and novel challenges on recombi -nant protein research .

关 键 词：亲和标签 重组蛋白 复合标签 多聚组氨酸标签 

分 类 号：Q503[生物学—生物化学]