右旋龙脑促进姜黄素抑制A375黑色素瘤细胞增殖的研究  

Potentiation of the Inhibitory Effect of Curcumin on A375 Melanoma Cell Proliferation by D-borneol

作　　者：吴萍[1] 陈建平[2] 李琳[1] 苏健裕[1] 曾峰 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640 [2]广东海洋大学食品科技学院,广东湛江524088 [3]江西吉安宇峰天然植物香料有限公司,江西吉安343000

出　　处：《现代食品科技》2016年第6期7-12,共6页Modern Food Science and Technology

基　　金：广东省自然科学基金面上项目(2014A030313265);广东省扬帆计划项目(201312H05);省部产学研结合项目(2013B020311009;2010A011200019;2011B010600029);华南理工大学中央高校基本科研业务费(2015ZZ050);广东省2015年度省公益研究与能力建设专项资金项目(2015A020209020)资助

摘　　要：本文运用MTT实验考察姜黄素和右旋龙脑分别在单独作用和联合作用时对黑色素瘤细胞A375存活率的影响,并进一步运用流式细胞术分析右旋龙脑联合姜黄素抑制黑色素瘤细胞A375增殖的原因。结果表明,姜黄素单独作用对A375细胞增殖有显著的抑制效应,而右旋龙脑单独作用对A375细胞增殖没有表现出明显的抑制作用。当用40μg/m L右旋龙脑预处理3 h,再与20μmol/L姜黄素联合作用A375细胞72 h后,其细胞存活率达到最低为10.93%,这比20μmol/L姜黄素单独处理时细胞的存活率降低了18.27%。进一步的流式结果表明,相比单独姜黄素处理,右旋龙脑与姜黄素联合处理后能明显提高Sub G1峰和G2/M比例,右旋龙脑(40μg/m L)与姜黄素(20μmol/L)联合作用黑色素瘤细胞A375后,其Sub G1和G2/M的含量分别从对照组的2.0%和16.4%升高到16.8%和40.1%,表明右旋龙脑与姜黄素主要是通过诱导细胞凋亡和G2/M阻滞来抑制黑色素瘤细胞A375增殖。An MTT assay was performed to investigate the effects of curcumin and d-borneol, alone or in combination, on the viability of A375 melanoma cells. The mechanism underlying the inhibitory effect of these compounds on melanoma A375 cell proliferation was determined by flow cytometry. The results indicated that curcumin alone could significantly inhibit A375 cell proliferation; however, d-borneol alone did not exhibit any obvious inhibitory effect on proliferation. When melanoma A375 cells were pretreated with 40 μg/m L d-borneol for three hours and then treated with 20 μmol/L curcumin for 72 hours, the lowest cell-viability rate（10.93%） was observed, which was 18.27% lower than the proliferation of cells treated with curcumin alone. Further, flow cytometric analysis indicated that compared with curcumin-alone treatment, a combination of curcumin and d-borneol could significantly increase sub-G1 peaks and G2/M phase ratios. When melanoma A375 cells were treated with a combination of d-borneol（40 μg/m L） and curcumin（20 μmol/L）, the populations of Sub G1 and G2/M increased to 16.8% and 40.1%, respectively, compared with 2.0% and 16.4% in the control group. These observations indicate that curcumin combined with d-borneol inhibits A375 cell proliferation by inducing apoptosis and G2/M cell cycle arrest.

关 键 词：姜黄素 右旋龙脑 抗增殖 联合作用 

分 类 号：R285[医药卫生—中药学]