蜜环菌生长培养基的优化及杂菌的分离鉴定  

Armillaria Growth Medium Optimization and the Separation of Mixed Bacteria Identification

作　　者：李再新[1] 唐文才[1] 

机构地区：[1]四川理工学院生物工程学院,四川自贡643000

出　　处：《食品研究与开发》2016年第12期155-158,共4页Food Research and Development

摘　　要：为提高蜜环菌产量,采用正交试验法对蜜环菌生长培养基中葡萄糖、酵母粉、维生素B13个组分的添加量进行优化,利用平板法分离出影响蜜环菌生长的主要杂菌并采用分子生物学的方法对污染菌株进行鉴定。试验结果表明:葡萄糖45 g/L、酵母粉9 g/L、维生素B10.05 g/L为最佳配方。分离出两株霉菌,经过鉴定,分别为橘青霉Penicillium citrinum t1、塔宾黑曲霉Aspergillus tubingensis t2。In order to improve the yield of Armillaria, glucose, yeast powder, vitamin B1 three components were optimized by the method of orthogonal experiment.The end of plate method was used to isolate the main bacterial and affect the growth of Armillaria strains of pollution by using molecular biology method. The results showed that the glucose 45 g/L, yeast powder 9 g/L, vitamin B10.05 g/L as the best formula. Two strains of the mold were isolated and identified, the results showed that the one was Penicillium citrinum t1, the other was As-pergillus tubingensis t2.

关 键 词：蜜环菌 正交试验 rDNA-ITS菌种鉴定 筛选 

分 类 号：S567.39[农业科学—中草药栽培]