紫花苜蓿病毒病症状类型及病原检测  

Symptoms and pathogen detection of alfalfa virus disease

作　　者：周其宇 梁巧兰[1] 韩亮[1] 

机构地区：[1]甘肃农业大学植物保护学院,草业生态系统教育部重点实验室,甘肃省草业工程实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《草业科学》2016年第7期1297-1305,共9页Pratacultural Science

基　　金：草业生态系统教育部重点实验室开放课题(CYZS-2011006);甘肃省财政厅项目(甘财教2013(116))

摘　　要：本研究为了探明甘肃省兰州市紫花苜蓿(Medicago sativa)侵染病毒病的症状类型、病原种类以及寄主范围,进行了田间调查和室内酶联免疫检测。田间调查确定了苜蓿病毒病在兰州市安宁区和皋兰县以及白银市景泰县均有发生,其中景泰县的发病率和病情指数均最高,分别为43.33%和26.08;共采样90份,检查30份,检查比例为30%。检测到的病原有苜蓿花叶病毒(AMV)、白三叶草花叶病毒(WCMV)、菜豆黄花叶病毒(BYMV)和豇豆花叶病毒(CPMV)4种,检出率分别为100.00%、50.00%、75.00%和75.00%,且存在复合侵染现象,复合侵染率达50.00%;寄主范围测定供试植物共6科17属18种,经测定,AMV可以侵染6科15种植物,WCMV可以侵染4科8种植物,BYMV和CPMV可以侵染3科7种植物,4种病毒可复合侵染6科14种植物。In order to investigate the symptoms,pathogen and host range of alfalfa virus diseases in Lanzhou City,Gansu Province,alfalfa virus diseases were investigated in field and pathogens were tested by DAS-ELISA. The results showed that the viral disease of alfalfa widely spread in Anning District and Gaolan County of Lanzhou and Jingtai County of Baiyin City and the highest incidence and disease index were 43. 33% and 26. 08,respectively,which were in Jingtai. A total of 90 sample were collected and 30 of them were detected which brought the detection ratio of 30%. Alfalfa mosaic virus（ AMV）,white clover mosaic virus（ WCMV）,bean yellow mosaic virus（ BYMV） and cowpea mosaic virus（ CPMV） were main viral pathogen which had detection rates of 100. 00%,50. 00%,75. 00%,75. 00%,respectively,and their mixed infection rate of 50. 00%. There were 6 families,17 genera and 18 species for host range testing.AMV can infect 6 families,15 species,WCMV can infect 4 families,8 species,BYMV and CPMV could infect 3 families,7 species,and four kinds of virus could co-infect 6 families,14 species of plants.

关 键 词：紫花苜蓿 病毒病 症状类型 寄主范围 

分 类 号：S435.4[农业科学—农业昆虫与害虫防治]