重组人血管内皮抑制素与白细胞介素-2对卵巢癌作用的实验研究  

Experimental study of function of recombinant human endostatin and interleukin-2 in the treatment of ovarian cancer

作　　者：于燕 张晓娜 亓健[2] 王晓燕[2] 毕春晖[3] 

机构地区：[1]莱芜市妇幼保健院生殖健康科,山东莱芜271100 [2]莱芜市人民医院妇产科,山东莱芜271100 [3]莱芜市人民医院介入科,山东莱芜271100

出　　处：《中国临床药理学杂志》2016年第14期1322-1324,共3页The Chinese Journal of Clinical Pharmacology

基　　金：山东省卫生厅一般计划基金资助项目(2009SDWB136)

摘　　要：目的研究重组人血管内皮抑制素与白细胞介素-2治疗卵巢癌的疗效。方法将80只卵巢癌裸鼠模型随机分为4组阴性对照组、阳性对照组及实验A、B组,每组各20只。阴性对照组未作特殊处理;阳性对照组予以尾静脉注射10 mg·kg^(-1)重组人血管内皮抑制素注射液,qd;实验A组予以肌内注射白细胞介素-2 1.0×10~6U·d^(-1),qd;实验B组予以转染白细胞介素-2基因的羊水干细胞,尾静脉注射0.2 m L微泡化,qd。4组疗程均为7周。每隔3 d测量一次肿瘤体积,末次处理后5 d剖取瘤块测体积、重量,计算抑瘤率。用TUNEL检测肿瘤细胞凋亡情况。结果 4组的肿瘤体积随时间逐渐增加,阴性对照组增加趋势明显高于阳性对照组和实验A、B组(P<0.05),但阳性对照组和实验A、B组比较差异无统计学意义(P>0.05)。治疗后,阳性对照组和实验B组的肿瘤重量分别为(878.3±30.5),(902.4±34.6)mg明显低于阴性对照组和实验A组的肿瘤重量(1554.7±45.3),(1484.6±41.4)mg(P<0.05),且阳性对照组的肿瘤重量和实验B组比较、阴性对照组的肿瘤重量和实验A组比较,差异无统计学意义(P>0.05)。阳性对照组和实验B组的抑瘤率和肿瘤凋亡细胞数均明显高于阴性对照组和实验A组(P<0.05),且阳性对照组和实验B组的抑瘤率和肿瘤凋亡细胞数比较、阴性对照组的抑瘤率和肿瘤凋亡细胞数和实验A组比较,差异无统计学意义(P>0.05)。结论转染白细胞介素-2基因的羊水干细胞可以抑制卵巢癌实体瘤的生长,促进肿瘤细胞凋亡。Objective To investigate the efficacy of recombinant human endostatin and interleukin- 2 in the treatment of ovarian cancer.Methods Eighty nude mices with ovarian cancer were randomly divided into negative control group,positive control group and the test A,B group,each group was 20 cases. Negative control group had no special treatment. Positive control group was treated with intravenous injection of 10 mg·kg^-1recombinant human endostatin injection,qd. Test A group was given intramuscular injection of interleukin- 2,1. 0 × 10~6U·d^-1,qd. Test B group was given stem cells of amniotic fluid transfected with interleukin- 2 gene,intravenous injection of 0. 2 m L microbubbles,qd.Four groups were treated for seven weeks. Tumor volume was mersured every 3 days,and the volume,weight,inhibition rate of all ovarian cancers＇ tumor block were mersured after 5 d of last handling. Tumor cellapoptosis was mersured by UNEL detection. Results Tumor volume increased with the time for four groups,and the increasing trend of negative control group was significantly higher than that of positive control group and test A,B group（ P〈0. 05）,but the increasing trend of positive control group and test A,B group had no statistical difference（ P〉0. 05）. After treatment,the tumor weight of positive control group and test B group was significantly lower than that of negative control group and test A group [（ 878. 3 ± 30. 5）,（ 902. 4 ± 34. 6） mg vs（ 1554. 7 ± 45. 3）,（ 1484. 6 ± 41. 4）mg,P〈0. 05]. The difference of the tumor weight between the positive control group and test B group was not statistically significant（ P〉0. 05）,so was that between the negative control group and test A group. Tumor inhibition rates and number of apoptotic cells in positive control group and test B group were significantly higher than those of negative control group and test A group（ P〈0. 05）. The difference of the tumor inhibition rates and number of apoptotic cells between the positive control group and test B g

关 键 词：羊水干细胞靶向治疗 白细胞介素-2基因 卵巢癌 

分 类 号：R97[医药卫生—药品]