润通茶质量标准研究  

Quality Standard for Runtong Tea

作　　者：檀巧婷[1] 黄爱文[1] 李蔚[2] 吴新安[2] 宋洪涛[1] 

机构地区：[1]南京军区福州总医院药学科,福建福州350025 [2]解放军105医院,安徽合肥230031

出　　处：《解放军药学学报》2016年第3期233-235,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的建立润通茶的质量控制方法。方法采用TLC法对润通茶中的大黄、木香进行定性鉴别;HPLC法测定制剂中大黄素、大黄酚的含量。色谱柱为依利特C18（4.6 mm×150 mm,5μm）,流动相为甲醇-0.1%磷酸（70∶30）,检测波长：254 nm。结果 TLC专属性强。大黄素、大黄酚分别在1.2～24.0、5.0～100.0μg·ml-1范围内线性关系良好。加样回收率分别为101.20%、99.18%,RSD分别为0.87%、1.04%。结论该方法可行,可有效控制润通茶质量。Objective To establish a quality standard for Runtong tea. Methods Rhei Radix et Rhizoma and Aucklandlae Radix in Runtong tea were identified by TLC. An HPLC method was established for the determination of emodin and ehrysophanol. Analysis was conducted on Elite C18 column（4.6 mm × 150 mm ,5 μm） with methanol-0.1% H3PO4 （70： 30） as the mobile phase at a detection wavelength of 254 nm. Results TLC was exelusive. Within the range of 1.2 - 24.0 μg .ml - 1, emodin presented a fine linear relationship, r = 0.9998 （ n = 5 ）. Within the range of 5.0 - 100.0 μg .ml -1, chrysophanol presented a fine linear relationship, r = 0.9999 （ n = 5 ）. The re- covery of emodin and chrysophanol was 101.20% and 99.18% respectively with an tlSD of 0.87% and 1.04% （ n = 6） respectively. Conclusion This method is reliable, which ean be used for quality control of Runtong tea.

关 键 词：润通茶 质量标准 TLC HPLC 大黄素 大黄酚 

分 类 号：R917[医药卫生—药物分析学]