五参安神胶囊的质量标准研究  

Improving Quality of Wushen Anshen Capsules

作　　者：金伟华[1] 陈华[1] 张明[1] 范开华[1] 蒲志强[1] 宋宗辉 龚应江 陈云舒 

机构地区：[1]成都军区总医院药剂科,四川成都610083 [2]川北医学院药学系,四川南充637100

出　　处：《解放军药学学报》2016年第3期253-256,共4页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的建立五参安神胶囊的质量标准。方法采用TLC法对石菖蒲、首乌藤、五味子进行定性鉴别;采用HPLC法测定五味子醇甲的含量,以十八烷基硅烷键合硅胶为填充剂,以甲醇-水(70∶30)为流动相,流速为1.0 ml·min^(-1),柱温为30℃,检测波长为250 nm。结果五味子、石菖蒲、首乌藤的TLC图均斑点清晰,阴性无干扰;五味子醇甲在1.24~39.6μg·ml^(-1)范围内线性关系良好(r=0.9999),平均回收率为100.89%,RSD为1.61%(n=9)。结论研究方法准确可靠,重复性好,可用于五参安神胶囊的质量控制。Objective To establish the quality standard for Wushen Anshen capsules. Methods Acori tatari- nowii rhizoma, Polygonum multiflorum caulis and Schizandra chinensis were qualitatively identified by TLC. The content of Schizandrin was determined by HPLC. The determination was performed on a ClS column with a mobile phase composed of methyl alcohol-water（70： 30）at a flow rate of 1.0 ml .min-1. The column temperature was 30 ℃ and the detection wavelength was set at 250 nm. Results TLC spots were fairly clear, and the negative samples showed no interference. The linear range of Schizandra methanol was 1.24 - 39.6 μg .ml-1 （ r = 0. 9999 ） with an average recovery of 100.89% , RSD = 1.61% （ n = 9 ）. Conclusion This method is accurate, reliable and reproducible, which can be used for quality control of Wushen Anshen capsules.

关 键 词：五参安神胶囊 TLC HPLC 五味子醇甲 

分 类 号：R927.2[医药卫生—药学]