山柰酚-3-O-鼠李糖苷抗炎作用的研究  

Inhibitory Effects of Kaempferol-3-O-rhamnoside on Inflammation

作　　者：陈曦[1] 陈斌[2] 李楠[1] 雍小兰[1] 

机构地区：[1]成都军区总医院临床药学科,四川成都610083 [2]第三军医大学新桥医院,重庆400037

出　　处：《解放军药学学报》2016年第3期260-262,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的研究山柰酚-3-O-鼠李糖苷的体内外抗炎作用。方法体外采用酶联免疫法检测山柰酚-3-O-鼠李糖苷对脂多糖刺激的小鼠单核巨噬细胞白血病细胞264.7细胞释放炎症细胞因子的影响;体内采用二甲苯致小鼠耳肿胀实验和角叉菜胶致大鼠足肿胀实验评价山柰酚-3-O-鼠李糖苷的抗炎作用。结果山柰酚-3-O-鼠李糖苷在50~200μg·ml^(-1)浓度范围内,可显著抑制脂多糖刺激的RAW264.7细胞炎症因子肿瘤坏死因子-α及白介素-6的释放,且对细胞活力无明显影响(P>0.05);山柰酚-3-O-鼠李糖苷200、400 mg·kg^(-1)对二甲苯所致小鼠耳廓肿胀以及角叉菜胶所致的大鼠足肿胀有明显的抑制作用(P<0.01)。结论山柰酚-3-O-鼠李糖苷具有一定的抗炎作用。Objective To investigate the anti-inflammatory effect of kaempferol-3-O- rhamnoside （KR） in vitro and in vivo. Methods TNF-α and IL-6 expressions of inflammatory eytokines in LPS induced RAW264.7 ceils treated with KR were measured with enzyme linked immunosorbent assay. The anti-inflammatory activities of KR in the xylene induced mouse ear edema model and earrageenin glue induced rat foot swelling model were observed. Results 50 -200 μg .ml- 1 of KR could significantly inhibit TNF-α and IL-6 levels in RAW 264.7 cells stimulated by LPS, but had no obvious effect on cell viability（ P 〉 0.05 ）. KR 200 and 400 mg .kg-1 significantly inhibited mouse ear edema caused by xylene and foot swelling caused by earrageenin （ P 〈 0.01 ）. Conclusion KR has anti-inflammatory effect.

关 键 词：山柰酚-3-O-鼠李糖苷 抗炎 细胞因子 

分 类 号：R965[医药卫生—药理学]