院内深部念珠菌感染菌种分布和耐药性分析  

Distribution and drug resistance analysis of nosocomial deep Candidainfection pathogens

作　　者：秦晓峰[1] 王英[1] 吴建华[1] 顾军[1] 

机构地区：[1]第二军医大学附属长海医院皮肤科,上海200433

出　　处：《中国真菌学杂志》2016年第3期166-168,共3页Chinese Journal of Mycology

基　　金：973项目(2013CB531602)

摘　　要：目的 了解我院深部念珠菌感染的类型、分布及耐药现状,指导临床用药。方法 采用常规方法分离培养真菌,对我院2012年6月-2014年5月培养出的念珠菌用科玛嘉念珠菌显色培养基和API 20CAUX进行鉴定,同时使用ROSCO纸片扩散法进行药敏试验。结果 共分离出念珠菌2 725株,其中白念珠菌最多（64.73%）,其次为热带念珠菌（13.64%）、光滑念珠菌（10.57%）和克柔念珠菌（2.35%）;念珠菌检出构成比以痰液最高（45.39%）,其次为中段尿（22.06%）和咽拭子（16.66%）;4种念珠菌对两性霉素B和5-氟胞嘧啶均表现出较高的敏感性。结论 院内深部念珠菌感染仍以白念珠菌为主,念珠菌对两性霉素B和5-氟胞嘧啶的耐药率较低,对唑类药物存在不同程度的耐药。Objective To understand the nosocomial deep Candida infection types,pathogenic strain distribution and the susceptibility to common antifungal agents,to provide reliable basis for clinical diagnose and treatment.Methods The fungi were isolated and cultured by using the conventional methods and identified by CHRO mager medium and API 20C AUX test.The drug susceptibility test was determined by ROSCO disc diffusion method.Results Totally 2 725 strains of Candida spp.were isolated,including Candida albicans （ 64.73 % ）, Candida tropicalis （ 13.64 % ）, Candida glabrata （ 10.57 % ） and Candida rusei （2.35 % ）. The Candida spp. were isolated from the sputum （45.39 %）, the urine （22.06 % ） and the throat swab （16. 66%） respectively. The four Candida species were sensitive to amphotericin B and 5-fluorocytosine. Conclusion Candida albicans was still the most common Candida spp. in the nosocomial deep Candida infections. The drug resistance rates of amphotericin B and 5-fluorocytosine were the lowest,while there was a genus difference in drug resistance of the az- ole drugs.

关 键 词：院内感染 念珠菌 深部 药敏试验 耐药性 

分 类 号：R519.3[医药卫生—内科学]