水飞蓟素对人骨肉瘤 Saos-2细胞的抑制作用及相关机制  

Inhibitory effect of silymarin on human osteosarcoma Saos-2 cells and its mechanism

作　　者：严晨[1,2] 邬亚华[3] 王子瑶[1,2] 殷嫦嫦[3] 殷明[1] 

机构地区：[1]南昌大学第二附属医院,江西南昌330006 [2]南昌大学研究生院医学部,江西南昌330006 [3]九江学院,江西九江332000

出　　处：《中国药理学通报》2016年第7期966-970,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81160226)

摘　　要：目的研究水飞蓟素对人骨肉瘤Saos-2细胞增殖、凋亡的影响,并探讨其可能的作用机制。方法设置对照组及不同浓度水飞蓟素组,作用人骨肉瘤Saos-2细胞。倒置相差显微镜观察细胞形态变化;CCK-8法测定细胞增殖;流式细胞仪检测细胞凋亡;Western blot检测ERK1/2、p-ERK1/2及cleaved caspase-3蛋白表达。结果水飞蓟素抑制Saos-2细胞增殖,呈时间及浓度依赖性;水飞蓟素诱导Saos-2细胞凋亡,呈浓度依赖性;水飞蓟素降低Saos-2细胞p-ERK1/2蛋白表达,增加Saos-2细胞cleaved caspase-3蛋白表达,呈浓度依赖性,而ERK1/2蛋白表达无明显变化。结论水飞蓟素能够抑制人骨肉瘤Saos-2细胞增殖及诱导其凋亡,其作用机制可能与抑制ERK信号通路及上调caspase-3蛋白表达有关。Aim To study the effect of silymarin on proliferation and apoptosis of human osteosarcoma saos-2 cells and to explore its possible mechanisms .Meth-ods Control group and different concentration groups of silymarin were set , and Saos-2 cells were treated with silymarin .Celluar morphologic changes were ob-served by inverted phase contrast microscope .Prolifer-ation of cells was tested CCK-8 assay .Apoptosis rate of cells was analyzed by flow cytometry . ERK1/2、p-ERK1/2 and cleaved caspase-3 protein expression were measured by Western blot .Results Silymarin inhibi-ted the proliferation of Saos-2 cells in a time-dependent and concentration-dependent manner . Silymarin in-duced the apoptosis of Saos-2 cells in a concentration-dependent manner . Silymarin decreased p-ERK1/2 protein expression and increased cleaved caspase-3 protein expression in a concentration-dependent man-ner, while ERK1/2 protein expression had no obvious change.Conculsions Silymarin can inhibit the prolif-eration of human osteosarcoma saos-2 cells and induce apoptosis of them , and the mechanism may be related to inhibition of ERK signaling pathway and upregulated caspase-3 protein expression .

关 键 词：水飞蓟素 骨肉瘤 增殖 凋亡 机制 ERK 信号通路 CASPASE-3 

分 类 号：R284.1[医药卫生—中药学] R329.24[医药卫生—中医学]